Cloning PCR products

Brett Beitzel brett_beitzel at msmtp.idde.saci.org
Mon Sep 26 13:00:55 EST 1994


I've used uracil DNA glycosylase (UDG) cloning to construct libraries
from products created by degenerate primers.  It is fairly simple and
straightforward.  You use primers modified with (CUA)n to amplify your
insert and vector then mix them in the presence of UDG.  The UDG degrades
the U in the CUA, leaving you with sticky ends.  You don't need to ligate
and you can transform this mix directly.
For more info see
	Nisson, Rashtchian, and Watkins  "Rapid and Efficient Cloning of Alu-PCR
Products Using Uracil DNA Glycosylase"  PCR Methods and Applications
1:120-123 (1991)

Brett Beitzel
brett_beitzel at msmtp.idde.saci.org
Cancer Therapy and Research Center
San Antonio, TX



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