Taq left at RT - gulp! -
Ann M. Yezerski
ayezersk at moose.uvm.edu
Tue Sep 27 16:48:39 EST 1994
Mark Siddall (mes at zoo.toronto.edu) wrote:
: Geez what a boo-boo...
: Accidentally left Taq out (covered though) at 25C for 24hr!!!
: Primers, buffersw, etc as well...
: Has anyone been foolhardy enough to have done this as well?
: Should I expect serious problems using this in the future? Or
: just chuck it all out?
Not quite the same situation, but...
I accidentally unplugged our frezzer while plugging in a computer (the
freezer was plugged into a traveling cart and I unplugged the cart). It
defrosted for 24 hours. I ran a PCR with extracts I hadn't even tried
yet, and, to my surprise, got the best results yet! Now, this does not
mean that you should go unplug your freezer. Also, I doubt that my Taq
ever reached room temperature, but that particular Taq is still in
working order two weeks and about 10 PCR's later. I suggest you try one
PCR with that Taq and see if it works. It's worth a try, and if the PCR
works, than you at least saved some money. Granted probably a couple of
"half-lives" have gone out of the Taq, and even if it does work, it
probably won't continue to do so for as long as it normally would.
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