PCR from cDNA library
parin at carrot.mcb.uconn.edu
Tue Sep 27 17:02:12 EST 1994
I'm trying to do PCR from cDNA library but I got a low yield.
The size of PCR product is about 1.7 kb. I used 10 ul of cDNA library as a
template in 50 ul reaction and used the extension time at 72 C about 3
min. By doing that I got very low yield. Any suggestion to improve the
yield would be appreciated.
Thank you in advance
Dept. of Molec & cell biol
U. of Connecticut
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