Air cyclers and sequencing

Randy P. Rasmussen Rasmussen at Bioscience.utah.edu
Tue Sep 27 14:22:09 EST 1994


In article <anderson-270994100001 at 128.252.181.14>,
anderson at pharmdec.wustl.edu (Eric C. Anderson) wrote:


> If you are using the ABI kits for autosequencing, the protocol
> specifically says to shield the reaction mixtures from light ... running
>the reactions in a thermocycler which relied on heat lamps would destroy
the >flourescent tags in no time flat.


I know of at least two different labs that use the ABI "Dye Deoxy"
sequencing kits and they have no problem with photo bleaching.

Have you actually tried doing reactions in an air cycler or are you just
guessing that it would be a problem?



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