promega wizard kits and the 373

TSRI Core Facility Bibbs at scripps.edu
Wed Sep 28 18:14:54 EST 1994


I talked with a Promega rep today and she told me that the problem with
the Wizard kits and the ABI 373 sequencer is that sometimes the fines from
the resin get in the DNA samples and inhibit the reaction.  Her suggestion
is to pellet the resin, remove the super and then ethanol ppt.  Has anyone
tried this with a sample that has failed?  We will try it tomorrow and
I'll post Friday.
Lisa Bibbs



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