Tricine for electrophoresis

Ding Ming ming at ahabs.wisc.edu
Wed Sep 28 11:03:41 EST 1994


In article <fsco-280994094825 at dalessandro.nci.nih.gov>, fsco at helix.nih.gov
(Francesco D'Alessandro) wrote:

> Do you have a protocol for Tricine gels to resolve and separate a 20 KDa
> protein? Or a reference where to find it? I also need the recipes for the
> buffers and conditions to run the gel.

The original tricine system was published by Schagger et al. in Anal.
Biochem., 166, 368-379.

> Does this kind of gels have any
> problem in transferring the proteins to nitrocellulose?

Not at all based on my experiences.

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Ding Ming
University of Wisconsin-Madison
Telephone: (608)265-3544     Fax: (608)262-7420
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