what's the best way to clone PCR products?

Cornelius Krasel krasel at alf.biochem.mpg.de
Wed Sep 28 06:25:41 EST 1994


Mike Preston (preston at warren.med.harvard.edu) wrote:
: 	For those of you who have had success direct cloning of PCR products do
: you have a step that gets rid of the Taq? 

Proteinase K digestion, as has been pointed out already several times by
diverse people.

--Cornelius.

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/* Cornelius Krasel, Abt. Lohse, Genzentrum, D-82152 Martinsried, Germany */
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/* "People are DNA's way of making more DNA." (Edward O. Wilson, 1975)    */



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