expression libraries

J Shepherd jms at festival.ed.ac.uk
Wed Sep 28 04:40:30 EST 1994


	I'm having a little trouble with probing an expression library;
I can't get consistant expression. I can produce great blue colour on an
X-gal test of intact gt-11, but I can't get the quoted 15% on a Ziplox
library.

	Do I need a different test for Ziplox?
	Do I assume that they are expressing?
	What effect does variation in the top agar have on induction?
	
	What are the biggest sources of 'noise' that anyone has managed
to pin down?

	Many thanks in advance; please e-mail replies, if any.

	Jason Shepherd.



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