35S-Met lifetime?

NICHOLAS THEODORAKIS ntheo at welchlink.welch.jhu.edu
Thu Sep 29 10:06:59 EST 1994


In article <1994Sep29.072250.20666 at news.unige.ch>,
Mike Morris <mike at divsun.unige.ch> wrote:
>How stable is 35S Met? should I aliquot it? Keep it at -80?

Yes to both. (even better would be to store under a nitrogen or argon 
atmosphere at -80, but I never bother.)

The QC sheet that comes with the label usually discusses the stability of 
35S-met at -20vs. -80. When its aliquoted and stored at -80, I usually 
find its ok for > one half-life (as assayed by labelling cells or by in 
vitro translation.)

					Nick



-- 
++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
			Nick Theodorakis
			ntheo at welchlink.welch.jhu.edu
			Johns Hopkins Medical School, Baltimore, MD



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