Cycle Sequencing Question

JEMERTON at BIOTECHNET.COM JEMERTON at BIOTECHNET.COM
Thu Sep 29 13:46:54 EST 1994


Hello -
I would like some pointers on cycle sequencing human genomic DNA (from blood)
CR.  We are trying to sequence D-Loop region of the mitochondria as well.
We have tried several concentrations of temp and primer.   We can get the
control to work (pGEM).  But not the genomic DNA.  Currently we are using
Promega Silver Sequence Kits.

Any tips?

Please direct your responses to KCMCELFRESH at BIOTECHNET.COM

Thanks - Kevin



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