Reticulocyte Lysate

[NICHOLAS THEODORAKIS]

In article <volcs-290994110825 at vockley7.mayo.edu>,
Sam Volchenboum <volcs at fermat.mayo.edu> wrote:
>In article <volcs-280994120713 at vockley7.mayo.edu>, volcs at fermat.mayo.edu
>(Sam Volchenboum) wrote:
>
>> I've been using alot of lysate for in vitro translation, and it's getting
>> really expensive - 1 ml = $110.  I would like to make my own, but don't
>> want to hassle with the animals (time, cost, etc.).  Does anyone know if
>> rabbit blood is commercially available?  I'm sure it's alot cheaper than
>> the lysate!  I cannot find it in the catalogs I have here.  Is it
>> reasonable to prepare retic lysate from commercially bought blood?
>
>
>
>Thanks to Frederick for answering my question.  Pel-Freez (1-800-643-4500)
>in Wisconsin sells reticulocyte rich blood, 25 ml for $44.  That will make
>lots of lysate, I'm sure!
>
>Sam

Try also Green Hectares (no kidding) in Oregon, Wisconsin (what's with 
Wisconsin and the rabbits?).  They sell retic. lysate, untreated (e.g., 
no hemin, non-nucleased) for about $5 - $10 per ml.  It's been awhile 
since I ordered, so I'm sorry that I can't remember the exact price.  I 
don't have the phone # with me now (I'm not at work), but if you want I 
can look it up later.

					Nick



-- 
++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
			Nick Theodorakis
			ntheo at welchlink.welch.jhu.edu
			Johns Hopkins Medical School, Baltimore, MD