Reticulocyte Lysate

[Sam Volchenboum]

In article <volcs-280994120713 at vockley7.mayo.edu>, volcs at fermat.mayo.edu
(Sam Volchenboum) wrote:

> I've been using alot of lysate for in vitro translation, and it's getting
> really expensive - 1 ml = $110.  I would like to make my own, but don't
> want to hassle with the animals (time, cost, etc.).  Does anyone know if
> rabbit blood is commercially available?  I'm sure it's alot cheaper than
> the lysate!  I cannot find it in the catalogs I have here.  Is it
> reasonable to prepare retic lysate from commercially bought blood?



Thanks to Frederick for answering my question.  Pel-Freez (1-800-643-4500)
in Wisconsin sells reticulocyte rich blood, 25 ml for $44.  That will make
lots of lysate, I'm sure!

Sam