Methods September 1994 Archives by author

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Starting: Thu Sep 1 00:35:05 EST 1994
Ending: Fri Sep 30 23:03:22 EST 1994
Messages: 1197

Antibiotic storage and shelf-life info wanted "Mary Colter ", BIO
RNA tips Stefan Gruenert (WCI)
pipette tune-ups
FMC SPIN BIND FOR DNA PREP? -sc2.sdsc.edu
Posting a new newswatcher group Labuser 1
Photoaffinity labelling via radioactive ATP analogues-help 191502931
DNA Polymerase "extendase" activity Enrique Jose Labadan Frio - SCBC - 3421101
DNA Polymerase "extendase" activity Enrique Jose Labadan Frio - SCBC - 3421101
DNA Polymerase "extendase" activity Enrique Jose Labadan Frio - SCBC - 3421101
Blue/white colony selection problem. Wises Namwat - SCMI - 3636183
Dig labeled probes 4700gbera at UMBSKY.CC.UMB.EDU
Cayman Chemical's EIA assay. 93huebner at wmich.edu
Liver RNA Prep Needed D.Greeve <DAEMON>
RNA Probe woes D.Greeve <DAEMON>
simian virus 40 we are looking for a plasmid map of pSV-7 as discribed by Colby and Shenk in 1982 (PNAS) at uni-muenster.de
Source of androgens DREW ARNOLD
Help-nucleic acids Abdelwahab_Ahmed at UQTR.UQuebec.ca
IMPORTANT! - BIOSCI at net.bio.net off-line Saturday PM. BIOSCI Administrator
UNSUBSCRIBING, BIOSCI ARCHIVES, ADDRESS DATABASE & BIOSCI FAQ BIOSCI Administrator
DNA from blood Dante Adorada
Effect of PEG on rxns Ashok Aiyar
Taq Pol and 3' primer mismatch? Ashok Aiyar
rna sequencing Mr. H.A. Al-Ghusein
gel retardation Mr. H.A. Al-Ghusein
PCR without protein extraction? Robert Allan
Plasmid preps for ABI 373A Ghis Allard
Plasmid preps for ABI 373A Ghis Allard
Semlike Forest Virus Allergan
Automated Sequencers Allergan
non-radioactive cycle sequencing Allergan
seq. power supplies Allergan
Sequencing Workshops Allergan
sequencing workshops Allergan
Reusing Ethidium Bromide Mike D Amstutz
Problems with Promega Wizard Preps? Mike D Amstutz
DATA MANAGEMENT of ABI373 Core Sequencing Facility Eric C. Anderson
Good mRNA Isolation Kit Eric C. Anderson
Columns for miniprep DNA Eric C. Anderson
ABI sequencing kit Eric C. Anderson
PCR: Promega Taq vs. Perkin-Elmer Taq Eric C. Anderson
RNA tips (DEPC and nucleic acid) Eric C. Anderson
Quantitating Radioactive label in DNA and RNA Eric C. Anderson
STOP ADVERTISING !!! Eric C. Anderson
Yeast Nitrogen Base?? Eric C. Anderson
Any softwares to draw plasmid maps? Eric C. Anderson
human genomic DNA PCR Eric C. Anderson
ABI sequencers with Genpak seq kits Eric C. Anderson
help with 5' race Eric C. Anderson
Non radioactive Northern Blot Procedure Eric C. Anderson
what's the best way to clone PCR products? Eric C. Anderson
What's the best mini-prep? Eric C. Anderson
Air cyclers and sequencing Eric C. Anderson
5' RACE Eric C. Anderson
Best methd. for PCR'in 10-15kb? Eric C. Anderson
ABI sequencing and Stratagene Taq Extender Eric C. Anderson
PCR PRIMER DESIGN HELP!! Eric C. Anderson
Ab to RFA or RPA 70 kD subunit Roger F. Anderson
Mammalian Cell Transfection FAQ? Goldberg Andrew
Efficient transfection into HepG2 The Answer
Is downward transfer REALLY good for Northern? Tracy Aquilla
site directed mutagenesis Tracy Aquilla
Sequencing Gel Spacer Plastic Tracy Aquilla
Promoter and DNA consensus-finding software Tracy Aquilla
NORTHERN BLOTS Tracy Aquilla
mRNA storage Tracy Aquilla
Buying Hyb. Oven/Roller Tracy Aquilla
PGEM accession numbers Tracy Aquilla
T7 Expression System Tracy Aquilla
Looking for mol bio software.... Tracy Aquilla
RT activity in Taq pol? Tracy Aquilla
site directed mutagenesis Tracy Aquilla
RT in taq Tracy Aquilla
Curiosity about Biotechnology/Molecular Biology Patents Tracy Aquilla
PEST-FIND algorithm Tracy Aquilla
Effect of PEG on rxns Tracy Aquilla
RT in taq Tracy Aquilla
PCR: Promega Taq vs. Perkin-Elmer Taq Tracy Aquilla
glycoprotein purification Tracy Aquilla
wanted: 2 umole scale synthesis of ribo-oligo (4mer) for under $150 Tracy Aquilla
Pisia vs Baculovirus, opinions wanted Tracy Aquilla
Re-diff. display problems Tracy Aquilla
Entrez Tracy Aquilla
protein overproduction in E. coli Tracy Aquilla
Tightly regulated E. coli Promoters Tracy Aquilla
RNA Probe woes Tracy Aquilla
Ribosomal RNA prep? Tracy Aquilla
DIG labeling Tracy Aquilla
PCR Clean up, 150bp frag??? Tracy Aquilla
High background on Northern Blot Tracy Aquilla
Non radioactive Northern Blot Procedure Tracy Aquilla
what's the best way to clone PCR products? Tracy Aquilla
Trizol reagent problems Tracy Aquilla
what's the best way to clone PCR products? Tracy Aquilla
what's the best way to clone PCR products? Tracy Aquilla
what's the best way to clone PCR products? Tracy Aquilla
ADVERTISING IN BIONET Tracy Aquilla
Best Way Clone PCR Products Tracy Aquilla
Perkin Elmer VS Thermolyne: Recommendations? Tracy Aquilla
what's the best way to clone PCR products? Tracy Aquilla
ADVERTISING IN BIONET Tracy Aquilla
Where to get shrimp alk. phosphatase? Tracy Aquilla
Oligo probe to Coding/non-coding strand - is there a preference? Tracy Aquilla
PCR with 3' mismatch Curt Ashendel
5' filling with Klenow Curt Ashendel
Why is tet-repressor system on two plasmids? Graham Atherton
Double tet expression system Graham Atherton
Cell cycle analysis kits Graham Atherton
Entrez Graham Atherton
Luciferase expression variability Graham Atherton
where is Cambridge Res. Biochem.? B.G.Macdonald
Bacterial Chromosomes and PFGE B.G.Macdonald
Looking for A Company; Australia. B.G.Macdonald
Inexpensive blotting paper? B.L.Cohen
CIP or BAP BEBLUM at ibc.unibe.ch
RNA tips BEBLUM at ibc.unibe.ch
sucrose gradients and protein separation AMRUT S BHOGLE
ultracentrifugation and proteins AMRUT S BHOGLE
unitcost-rflp/pcr Ann Baker
Anti peptide Abs? J.J. Baker
(none) Laura Anne Bannister
study of prostate tumors Ryan Bartel
unsubscribe Richa Behari
Reusing Ethidium Bromide Brett Beitzel
DIG labeling PCR probes Brett Beitzel
DIG labeling PCR probes II Brett Beitzel
Cloning PCR products Brett Beitzel
Taq left at RT - gulp! - Brett Beitzel
faint bands in PCR Dr. M.V. Bell
Electroporation Improvements Ben-Sapiens
Electroporation Improvements Ben-Sapiens
Electroporation optimal DNA conc.? Michael Benedik
Where to get shrimp alk. phosphatase? Michael Benedik
Plasmid preps for ABI 373A Lisa Bibbs
subscribe Lisa Bibbs
Centri-sep re-use Lisa Bibbs
Cycle sequencing - no product Lisa Bibbs
SEQUENCING OF PCR PRODUCTS?? HMMMMMMMMMMM Lisa Bibbs
ABI sequencing artifact Lisa Bibbs
Oligo synthesisers: Millipore Expedite vs Beckman Oligo 1000 Lisa Bibbs
FMC SPIN BIND FOR DNA PREP? Lisa Bibbs
unsubscribe Lisa Bibbs
Urgent! Fax-Number from Vical Inc. needed! Inst. f. Biochemistry
Turner Fluorimeter David F. Bishop
amino acid frequency Shaun D. Black
Apotag Kit (cell death kit) Vivien Bonazzi
seed cDNA libraries Peta C. Bonham-Smith
Sequencing Workshops Nancy Bowers
Clontech's Transformer Kit- Help please Dave Boyd
Looking for A Company; Australia. Dr Stuart Boyer
MJR 96-well PTC-1 machine: suitable microtitre plates? Toby Bradshaw
Electroporation of COS: How to? Tomas Bratt
How to make random blunt end cuts in a plasmid? BrianC4208
Yeast Nitrogen Base?? Klara Briknarova
Southern Hybridization - Strigency Question Stuart Brown
Sequencing insert from Lambda gt10 vector- HELP! Stuart Brown
two hybrid system and differential display Karen Browning
genetics research Wolfram Brune
lambda/HindIII John Brunstein
Eastern blotting! John Brunstein
STOP ADVERTISING !!! John Brunstein
Sephadex G-50 Maureen Bunger
Northern blots, which is the best membrane?? Thomas Burglin
Northern blot memranes, summary Thomas Burglin
Columns for miniprep DNA Jon Burrows
In Vivo Excision of ZapII Jon Burrows
RNA Probe woes Jon Burrows
PCR Clean up, 150bp frag??? Jon Burrows
Any softwares to draw plasmid maps? Timothy Bushnell
ABI sequencing kit Tim Buss
CIP or BAP Tim Buss
PCR from serum C.J. Hussussian, MD
pcr yield Mr. C.S.Wilding
Need restriction map of pT7.7 CBA0034 at uabdpo.dpo.uab.edu
BAC vectors CHAZ
Southern Hybridization - Strigency Question CMS
re: PCR from intact bacterial cells CORROCHANO PELAEZ, LUIS M.
Re: Taq left at RT - gulp! - CORROCHANO PELAEZ, LUIS M.
.01um pore filters CURTIS at UTMSI.ZO.UTEXAS.EDU
Subcloning PCR Andrew Cannons
Reusing Ethidium Bromide Dave Carmean
Trehalose for tissue/DNA preservation Dave Carmean
Does anyone have experience with LONG PCR? Kelly Carpenter
PEST-FIND algorithm Darrick Carter
Yeast expression library ?? Darrick Carter
Transients-DMSO shock. George W Chacko
OKT8 (biotinylated) George W Chacko
Ribosomal RNA prep? Daniel Chagnovich
LA PCR Mic Chaudoir
What's the best mini-prep? Mic Chaudoir
program for Motif identification? Daniel S. Chen
Help for IGF1, IGF2, and receptor's probes and antibodies Jun Chen
antibody to SOD Shao-Yu Chen
TGF beta related Shu-Chih Chen
DNA Polymerase "extendase" activity Joshua Cherry
RPA Assay kits Chutney
RNA tips Chutney
RPA Assay kits Chutney
Fuji film sensitivity loss Chutney
2-D protein gels Chutney
RNA Probe woes Chutney
Help with RPA on tissue lysates Chutney
Trizol reagent problems Chutney
Trizol reagent problems Chutney
MW Markers for RNA gels... Chutney
Problem getting JM110 competent Duncan Clark
list of E. coli genotypes Duncan Clark
RT in taq Duncan Clark
'Easy' Blue/white cloning Duncan Clark
Recipe for linear polyacrylamide Duncan Clark
Blue/white colony selection problem. Duncan Clark
expression/induction depends of container Duncan Clark
T-tailing for PCR product ligation Duncan Clark
Electroporation Improvements Duncan Clark
Linear polyacrylamide as an alternative to glycogen carrier Duncan Clark
what's the best way to clone PCR products? Duncan Clark
@#! smears on PCR ??? Duncan Clark
Taq left at RT - gulp! - Duncan Clark
ABI sequencing kit Kevin Clark
ABI sequencing kit Kevin Clark
ABI sequencers with Genpak seq kits Kevin Clark
capillary electrophor. - sensitivity w/fluorescence?? Neil Clarke
SYBR Green I Michael Coady
PCR inhibitors in insect tissue? Andrew Cockburn
RNA freezing Andrew Cockburn
Mitochondrial genome size determination Andrew Cockburn
Determination of genome size (Cot curves) Andrew Cockburn
periplasm signal sequences Robert C. Colgrove
WANTED: Old liquid scintillation counters Matthew J. Combs
looking for nucleosidase Pierre Commercon
Blood DNA Pierre Commercon
5 Kb by RT-PCR Pierre Commercon
Re-using SYBR Green? Julie Constable
Subcloning PCR Juan Antonio Contretras
Reversible PCR termination possible? Jim Cook
Pisia vs Baculovirus, opinions wanted Seth Corey
Quantifying mouse IFNAR??? Mary Coussons-Read
Quantifying mouse IFNAR??? Mary Coussons-Read
Quantifying mouse IFNAR revisited... Mary Coussons-Read
RNA extraction from fixed tissue? Mary Coussons-Read
direct transfection of PCR products Seth Daniel Crosby
needed Cell/Molecular Biologist Seth Daniel Crosby
PCR from intact bacterial cells Seth Daniel Crosby
Loop-out PCR Seth Daniel Crosby
Deprobing problem Deborah Croteau
Mitochondrial replication Deborah Croteau
Tricine for electrophoresis Francesco D'Alessandro
CIP or BAP Johnny D
amber ampicillin resistance (pivx users) Johnny D
Ecoli origin D=Piket%buro%preklin at Asklepios.med.vu.nl
remove my name from the list MAHER DERBEL
Li-cor DNA-Sequencer Bill Oetting DGM-IHG
Help with PCR Scott Wildenberg DGM-IHG
modified oligos Francois DRAGON
Need info: any vectors harbouring SP1 or pRB Lee Dae-Youp
help with protein expression Bipin K Dalmia
UV Protein Determination. Bipin K Dalmia
arabidopsis RNA prep needed Richard Dana
Liver RNA Prep Needed Richard Dana
ADVERTISING IN BIONET Richard Dana
DNA from blood Richard Dana
Phage DNA storage temp. Joshua Daniels
retinol, cholecalciferol, vitamin B12 David J. Austin, Ph.D.
Searching for silent mutations... David J. Austin, Ph.D.
Lost DNA after precipitation David L. Haviland, Ph.D.
NORTHERN BLOTS David L. Haviland, Ph.D.
PEG debate David L. Haviland, Ph.D.
Disappearing Clones during Library Screening Jeff Dean
Microwave restriction digests Koen Dechering
help with 5' race Gill Diamond
Microwave restriction digests John Dixon
Microwave restriction digests John Dixon
graphics from GCG programs Tom Doak
testing Dorothea.Smith
microbial activity in soil Dorothea.Smith
More Densitometry software? Mr TG Downing
Microwave Miniprep - protocol? Mr TG Downing
More Densitometry software? Mr TG Downing
MW Markers for RNA gels... Virginia Dress
Pisia vs Baculovirus, opinions wanted Virginia Dress
Reverse Northern Blot? Dr. YL Du
Promega on the Internet Dr. YL Du
Eastern blotting! Nigel Eastmond
reusing agarose gels Nigel Eastmond
NIH Imaging software Nigel Eastmond
RNA tips (DEPC and nucleic acid) Sean Eddy
Beware pGEM-T Phil Emery
e.coli transformation question the End
Spontaneous Spectinomycin resistance ? the End
Spontaneous Spectinomycin resistance ? the End
site directed mutagenesis the End
Precip. DNA from agarose "squeeze" ? the End
Tightly regulated E. coli Promoters the End
Li-cor DNA-Sequencer Armin Ensser
info about suicide vectors systemsIs2 PANIAGUA SOLIS JORGE FERNANDO
promega wizard kits and the 373 TSRI Core Facility
DNA sequencer TSRI Core Facility
Automated Sequencers TSRI Core Facility
Help with PCR Tom Fagan
Where to get ppGpp?? Hoosier Habs Fan
Bacterial Chromosomes and PFGE Mark Farinha
p13suc1-Sepharose Heidi Feiler
Recommendations for light level protein detection Dr. D.G. Fernig
Quantitative PCR help Sergi Ferrer
Help wanted, RNA isolation Jackie Fetherston
Two-Hybrid: Looking for S.cerevisiae Activation-Domain Library Seth Findley
Phage display expression library H. David Fischer
Who has this stuff? Parke K. Flick
Where to get shrimp alk. phosphatase? Parke K. Flick
raising ANTIBODIES with fusion proteins?? Riccardo Fodde
program for Motif identification? Brian Foley
TA cloning doesn't work if a certain base is at 5' end of primer? Brian Foley
@#! smears on PCR ??? Brian Foley
Promega Taq vs. Perkin-Elmer Taq Patricia Foster
sequencing John E. Fox
where is Cambridge Res. Biochem.? John E. Fox
Polypeptides John E. Fox
Cheap Peptides (sm scale)? John E. Fox
Cheap Peptides (sm scale)? John E. Fox
Automated Sequencers John E. Fox
Secretion helper vector for E.coli Priyal A. De Zoysa now at Royal Free
Looking for Yeast Genetic Stock Priyal A. De Zoysa now at Royal Free
Source for hygromycin resistance plasmid Marion Freistadt
PCR from phage Brian Fristensky
Sol 8 Transfection? Jody G.
retinol, cholecalciferol, vitamin B12 Victor Jimenez G.
Extraction of DNA from agarose gels GABRE at wwg3.uovs.ac.za
teaching molecular biology lab to undergrad students Prasit Palitpolkanpim - GRSO
Microwave restriction digests Petr GRUZ
High background on Northern Blot GUT at QUCDN.QueensU.CA
Ribosomal RNA prep? GWDGV1::KCHOWDH
Source for hygromycin resistance plasmid GWDGV1::KCHOWDH
Rf: RE; touchdown PCR G Gallagher
amylase and cellulase Mark A Gallo
SSCP help Frederick Garbrecht
2 bands instead of 1 Frederick Garbrecht
PCR smear Frederick Garbrecht
@#! smears on PCR ??? Frederick Garbrecht
SSCP question Frederick Garbrecht
CDM8 sequence Georges Gaudriault
Sequencing using infrared tags Peter Gegenheimer
pCp (NOT the drug) Peter Gegenheimer
Tightly regulated E. coli Promoters Peter Gegenheimer
Li-cor DNA-Sequencer Peter Gegenheimer
RNA tips (DEPC and nucleic acid) Peter Gegenheimer
T7 RNA Polymerase Peter Gegenheimer
Tightly regulated E. coli Promoters Peter Gegenheimer
Other 'merlin' celite preps out there? Charlie Wright Genetics
Microwave restriction digests Charlie Wright Genetics
pipette tune-ups Farid John Ghadessy
calibrating gilsons Farid John Ghadessy
Fusion protein was trapped in the cell pellet Marc A. Goldstein
expression/induction depends of container Marc A. Goldstein
shearing DNA to 1kb size Michael Gorry
Treponema phagedenis DNA Jerry Grandoni
PCR screening: False positives Dr. W. Greenhale
RNA tips Darren Greeve
CAT mRNA J Grimbergen
G50 to remove unicorporated isotope Brian Grimwade
T7 RNA Polymerase Duke Groebe
COMT reaction using erythrocytes Ilonka Guenther
transfection of monocytic cells Markus Guetlich
transfection of monocytic cells Markus Guetlich
Any softwares to draw plasmid maps? Xin Guo
list of E. coli genotypes HARDIES at THORIN.UTHSCSA.EDU
Effect of PEG on rxns HARDIES at THORIN.UTHSCSA.EDU
black dots on DNA seq. gels HARDIES at THORIN.UTHSCSA.EDU
differential display PCR HARDIES at THORIN.UTHSCSA.EDU
SSCP help HARDIES at THORIN.UTHSCSA.EDU
(none) HARDIES at THORIN.UTHSCSA.EDU
G50 to remove unicorporated isotope HARDIES at THORIN.UTHSCSA.EDU
2 bands instead of 1 HARDIES at THORIN.UTHSCSA.EDU
SEQUENCING OF PCR PRODUCTS??? HMMMMMM HARDIES at THORIN.UTHSCSA.EDU
Dig labeled probes HARDIES at THORIN.UTHSCSA.EDU
RNA tips (DEPC and nucleic acid) HARDIES at THORIN.UTHSCSA.EDU
protein overproduction in E. coli HARDIES at THORIN.UTHSCSA.EDU
Diff. display problems HARDIES at THORIN.UTHSCSA.EDU
Help with PCR HARDIES at THORIN.UTHSCSA.EDU
High background on Northern blot HARDIES at THORIN.UTHSCSA.EDU
use of BSA in... (why air kills) HARDIES at THORIN.UTHSCSA.EDU
LA PCR HARDIES at THORIN.UTHSCSA.EDU
Re. Southern hyb. - Stringency question HARDIES at THORIN.UTHSCSA.EDU
Southern hyb. - Stringency question HARDIES at THORIN.UTHSCSA.EDU
cloning PCR products HARDIES at THORIN.UTHSCSA.EDU
PCR for radiolabelling probes HARDIES at THORIN.UTHSCSA.EDU
Km of rest. enzymes. HARDIES at THORIN.UTHSCSA.EDU
SSCP question (cold is better) HARDIES at THORIN.UTHSCSA.EDU
PCR primer design (r. site exten.) HARDIES at THORIN.UTHSCSA.EDU
PC12 cells- how to culture Allison Haggarty
COMPETENT CELLS PROTOCOL!!! Harry Hahn
RNA sequencing with RTase? Harry Hahn
Affinity column needed.. Kim Hak-Sung
about filber digestive GENE(s) Andre Hamel
Promega on the Internet Jutta Hammermueller
ligation R Hanemaaijer
DATA MANAGEMENT of ABI373 Core Sequencing Facility Richard R. Hardy
ABI sequencing kit Richard R. Hardy
DATA MANAGEMENT of ABI373 Core Sequencing Facility Richard R. Hardy
Columns for miniprep DNA Richard R. Hardy
ABI sequencing kit Richard R. Hardy
In situ question Uwe Harmening
Extraction of DNA from agarose gels Jane Harrison
SuperScript II RNase H- RT Harry_Au at SOM-LRC.UCSD.EDU
pipette tune-ups Yasha Hartberg
method(s) to measure ATP Yasha Hartberg
calibrating gilsons Yasha Hartberg
protein overproduction in E. coli Yasha Hartberg
Isolation of RNA from liver Carla Hass
Ultra-low Freezers Carla Hass
Subcloning PCR Randy Haun
CAT mRNA Randy Haun
what's the best way to clone PCR products? Randy Haun
Re. RNA Storage. J.P. Hays
Tightly regulated E. coli Promoters Philip Heacock
Glowing BLUE DNA Gels Philip Heacock
Oligo dT Too Heng-Phon
rescue dead stock Paul N Hengen
SYBR Green I Paul N Hengen
Plasmid preps for ABI 373A Paul N Hengen
membrane proteins Paul N Hengen
Frequently Asked Question (FAQ) list Paul N Hengen
Does anyone have experience with LONG PCR? Paul N Hengen
Glowing BLUE DNA Gels Paul N Hengen
homemade receipt for ECL reagents Your Name Here
Subcloning PCR Bernard Heymann
Subcloning dirty PCR products Bernard Heymann
Where to get shrimp alk. phosphatase? Bernard Heymann
Effect of PEG on rxns Robert Hindges
PCR: Promega Taq vs. Perkin-Elmer Taq Annette C. Hollmann
RNA tips Annette C. Hollmann
SEQUENCING OF PCR PRODUCTS?? HMMMMMMMMMMM Annette C. Hollmann
Are Ab's phosphorylated? Annette C. Hollmann
Thrmobin inhibition/removal Gene Holowachuk
Negative Cooperativity Louis Hom
Are Ab's phosphorylated? Louis Hom
On-line source for genotypes? Peter Horn
More Densitometry software? Robert Horton
graphics from GCG programs Robert Horton
pcr Robert Horton
Blood DNA Robert Horton
Double tet expression system David S. Huen
graphics from GCG programs Olav Hungnes
DNA Polymerase "extendase" activity Olav Hungnes
DNA Polymerase "extendase" activity Olav Hungnes
Oligo dT Olav Hungnes
TA cloning doesn't work if a certain base is at 5' end of primer? Olav Hungnes
>>>>URGENT : IN VITRO TRANSCRIPTION <<<<< CJF 90/10 INSERM
Extraction of DNA from agarose gels CJF 90/10 INSERM
>>>>Help on In-Vitro Transcription<<< CJF 90/10 INSERM
Ribosomal RNA prep? RICHARD ISBRUCKER
DNA cruciforms and Mg2+ in vivo ? Horst Ibelgaufts
black dots on DNA sequencing gels? Susan E Ingraham
PEG debate JAB5 at VAX.YORK.AC.UK
Cycle Sequencing Question JEMERTON at BIOTECHNET.COM
Quantitating Radioactive label in DNA and RNA JFrank777
Lg Scale Fermentation JFrank777
Spin-Elution and ppt. of DNA JFrank777
Ist strand cDNA purification. JOEDAG
Qui A ITABAJARA DA SILVA VAZ JUNIOR
Laser densitometers Jack M. Bernstein, M.D.
HELP-- TRANSFECTION GRADE PLASMID DNA Jack M. Bernstein, M.D.
pCp (NOT the drug) Jack M. Bernstein, M.D.
Km of restriction enzymes Bill Jack
Fungus in Microfuge tubes Neeraj Jain
HELP-- TRANSFECTION GRADE PLASMID DNA Sue Jeffrey
Semlike Forest Virus Sue Jeffrey
Detection of rRNA methylation Donna Jensen
ligating small fragments into large vectors Jack Jenuth
artifact bands on sequencing gels L. Fred Jerva
DNA-affinity columns Joanne
Microtiter Plates Joe M. SandyFaulkner, Jr.
Protein electroelution from SDS-page JoeJoe
Good mRNA Isolation Kit JoeJoe
RNA tips JoeJoe
where is Cambridge Res. Biochem.? daj (David Johnston)
Cheap Peptides (sm scale)? Chris Jones
Looking for pGK12 plasmid or creators Derek Jones
Looking for pGK12 Derek Jones
Hexamer Primer Walking Sequencing Dr. M.D. Jones
Gapdh RT-PCR Probe Dr. M.D. Jones
In situ question P. Jones
Lost DNA after precipitation Zophonias O. Jonsson
T7 Expression System Zophonias O. Jonsson
Subcloning dirty PCR products Zophonias O. Jonsson
Subcloning dirty PCR products Zophonias O. Jonsson
Tightly regulated E. coli Promoters Zophonias O. Jonsson
Amplification of plasmids NOT with Chloramphenicol Zophonias O. Jonsson
PCR directly from phage Ron Kagan
human genomic DNA PCR Ron Kagan
Method for Touchdown PCR Ron Kagan
Method for Touchdown PCR Ron Kagan
UV Protein Determination. Ron Kagan
T-tailing for PCR product ligation Ron Kagan
Downward DNA transfer? Ron Kagan
Phage display expression library Kah-Tong, Seow
STOP ADVERTISING !!! Stefan Kahlert
SEQUENCING OF PCR PRODUCTS??? HMMMMMM Kris Kalra
5' filling with Klenow Jonathan M Kaplan
Linear Plasmid Ali Karami
Linear Plasmid From Borrelia Ali Karami
DIG labeled probes Kath.Nesbitt at plant.utas.edu.au
DIG labeling Kath.Nesbitt at plant.utas.edu.au
Efficient transfection into HepG2 Yoshiki Kawabe
black dots on DNA sequencing gels? Martin Kennedy
5' filling with Klenow Martin Kennedy
5' filling with Klenow Martin Kennedy
Pefabloc (Was: membrane proteins) Martin Kennedy
inactivation of phosphatase Martin Kennedy
use of BSA in EMSA supershifts Chong-Yee Khoo
Fast thermocycler In C. Kim
SYBR Green I, more exper Jun Kim
Catalogs for microscopy? Jun Kim
Air cyclers and sequencing Jun Kim
RT activity in Taq pol? Klaus.Matthaei at ANU.EDU.AU
embryonic stem cells Klaus.Matthaei at ANU.EDU.AU
Quantitating Radioactive label in DNA and RNA Klaus.Matthaei at ANU.EDU.AU
Liver RNA Prep Needed Klaus.Matthaei at ANU.EDU.AU
Quantitating Radioactive label in DNA and RNA Klaus.Matthaei at ANU.EDU.AU
RNA Probe woes Klaus.Matthaei at ANU.EDU.AU
Glowing BLUE DNA Gels Klaus.Matthaei at ANU.EDU.AU
Taq left at RT - gulp! - Klaus.Matthaei at ANU.EDU.AU
PCR w/ capillary tubes Klaus.Matthaei at ANU.EDU.AU
Hair Follicle DNA Fingerprinting Ann Kleinschmidt
Fax number of Clontech Hannes Klump
Air cyclers and sequencing Eric Kofoid
blunt end ligation Ryszard Kole
RT-PCR Ryszard Kole
pipette tune-ups John Korte
plasmid minipreps Sasha Kraev
extraction from agarose gels Sasha Kraev
gt10 insert sequencing Sasha Kraev
Subcloning dirty PCR products Cornelius Krasel
Quantitating Radioactive label in DNA and RNA Cornelius Krasel
Epitope-Tagging and EM Cornelius Krasel
ADVERTISING IN BIONET Cornelius Krasel
what's the best way to clone PCR products? Cornelius Krasel
Ultra-low Freezers Cornelius Krasel
5'->3' InhibitACE Cornelius Krasel
Making buffer Min Kuan
UV Protein Scanning. Min Kuan
UV Protein Determination. Min Kuan
UV Protein Determination. Min Kuan
mLFA-3 Ab Mr. M. Kuiper
Subtractive cDNA libraries Peter Kulmburg
Broccoli question LEES at SASK.USASK.CA
Turner Fluorimeter LEES at SASK.USASK.CA
Downward DNA transfer? LEES at SASK.USASK.CA
Methylene blue staining for DNA? LEES at SASK.USASK.CA
Dinucleotide Repeat PCR LICHY at EMAIL.AFIP.OSD.MIL
method(s) to measure rATP LKM100F at ODUVM.BITNET
RNA tips (DEPC and nucleic acid) LKM100F at ODUVM.BITNET
35 mm slides of gels - help LKM100F at ODUVM.BITNET
Method for Touchdown PCR LOGAND
Rf: RE; touchdown PCR LOGAND
Rf: Measuring Intracellular Calcium Concentration LOGAND
Rf: 2-D protein gels LOGAND
Desperately seeking Schering Agrochemicals LOGAND
Questions on failed T/A cloning LOGAND
question for Aussies only LOWEN,Darren
Taq DNA Pol and frameshifts Hanson Lab
method(s) to measure rATP Hanson Lab
Curiosity about Biotechnology/Molecular Biology Patents Hanson Lab
Fusion protein was trapped in the cell pellet S.A. Benson Lab
Fusion protein was trapped in the cell pellet S.A. Benson Lab
display phage inactivation Ward Lab
Columns for miniprep DNA Carlisle Landel
CIP or BAP Carlisle Landel
RNA Probe woes Carlisle Landel
Taq Pol and 3' primer mismatch? Carlisle Landel
Sequencing insert from Lambda gt10 vector- HELP! Carlisle Landel
What video-camera for DNA-Gels????? Andreas Langsdorf
5' RACE Tom K. Lannom
Bacterial Chromosomes and PFGE Brian_D Lanoil
More Bacterial Chromosomes and PFGE Brian_D Lanoil
16S RNA E-mail server? Niels Larsen
black dots on DNA sequencing gels? Stephen R. Lasky
Reuse of Qiagen columns Stephen R. Lasky
gel pull off Stephen R. Lasky
differential display PCR Stephen R. Lasky
RNA tips Stephen R. Lasky
Columns for miniprep DNA Stephen R. Lasky
membrane proteins Stephen R. Lasky
transfection of monocytic cells Stephen R. Lasky
Ultra-low Freezers Stephen R. Lasky
T7 RNA Polymerase Yoon Lee
mouse evx 1 probe? Rachelle Leger
help with 5' race Rachelle Leger
need a good DNA sequencing service Kaiser Ham Lice
sephadex G-50 Lauri Lintott
Screening Phage Bank With Degenerate Oligo Eric Lippay
what's the best way to clone PCR products? Eric Lippay
pcDNA3 Frosty Loechel
Circular Dichroism Mark Jason Logan
Circular Dichroism Help! Mark Jason Logan
Best methd. for PCR'in 10-15kb? Rob Logan
SYBR Green I, more exper Jeanne Loring
Subcloning PCR Jeanne Loring
SYBR Summary-Molecular Dyna Jeanne Loring
SYBR Summary Jeanne Loring
SYBR Summary-hanzel@mdyn.co Jeanne Loring
T7 Expression System Patric Lundberg
protein overproduction in E. coli Patric Lundberg
Thrmobin inhibition/removal Patric Lundberg
Promega on the Internet Peter Lundberg
RNA Probe woes Jukka Luoma
Short eukaryotic promoter? Alan Lyons
Mammalian cell transfection? Alan Lyons
Secondary screening after differential display? Alan Lyons
Need restriction map of pT7.7 M.A.Schmidt
subscription MBC4917 at ACS.TAMU.EDU
Promega on the Internet MEG-CNR
Seq Gel Spots DAVID MITCHELL
Seq Gel Spots" DAVID MITCHELL
Seq.power supply SEPPO KAIJALAINEN MMETT
Info about AFLP NICOLA LA PORTA MMKAB
Help with RPA on tissue lysates MUNGO at UVVM.UVIC.CA
RT in taq NEAL MUSTO
Source of androgens NEAL MUSTO
RE; touchdown PCR NEAL MUSTO
Biotinylated proteins NEAL MUSTO
SEQUENCING OF PCR PRODUCTS?? HMMMMMMMMMMM David MacHugh
primers with dI vs mixed bases Emma Macfarlane
Removal of Dna Probes Emma Macfarlane
Reusing Ethidium Bromide Emma Macfarlane
Immunoaffinity Columns Emma Macfarlane
Reuse of Qiagen columns Lakshmi Madabusi
Best Way Clone PCR Products Dave Mahaffey
Blue/white colony selection problem. Rafa Maldonado
Spontaneous Spectinomycin resistance ? Rafa Maldonado
help with protein expression Rafa Maldonado
list of E. coli genotypes Rafa Maldonado
Leader Peptides/Attenuators Rafa Maldonado
More Bacterial Chromosomes and PFGE Rafa Maldonado
to T. Bickle Rafa Maldonado
PCR Clean up, 150bp frag??? Rafa Maldonado
G50 to remove unicorporated isotope Rafa Maldonado
Help- getting out 0.5 kb insert from plasmid. Mandalaparthy
Help- with lge scale phage prep Mandalaparthy
Sequencing insert from Lambda gt10 vector- HELP! Mandalaparthy
Ultra-low Freezers Brian Margolin
Gel Supershifts Dominique Marilley
DATA MANAGEMENT of ABI373 Core Sequencing Facility Steve Marsh
graphics from GCG programs Craig Marshall
subscribe Richard Kenneth Bruce Martel
smear on PCR gels Martin.Gossen at RUBA.RZ.ruhr-uni-bochum.de
Oligo synthesisers: Millipore Expedite vs Beckman Oligo 1000 Dr. Jeremy Martinson
Oligo synthesisers: Millipore Expedite vs Beckman Oligo 1000 Dr. Jeremy Martinson
gilson calibration .... how? Massimo
ABI sequencing kit Viraj Master
Fast thermocycler Viraj Master
What video-camera for DNA-Gels????? Viraj Master
Microwave restriction digests Viraj Master
Fast thermocycler Viraj Master
Problems with 1.5 ml Eppendorf tubes Raffaele Matteoni
Any experience with Soccorex pipettors John H McDonald
Km of restriction enzymes John H McDonald
Air cyclers and sequencing John H McDonald
Ribosomal RNA prep? John McDougall
in vivo footprinting Steve McKinney
Recommendations for light level protein detection? Kenneth J McNeil
Cre/lox vectors Dr Johnjoe Mcfadden
Eastern blotting! Dan Mcleod
Silver Staining of GT gels Lisa A. Megna
single-step RNA method and sodium acetate Daniel Meier
PCR directly from phage Harry Menegay
RNA extraction from tissue in OCT embedding compound Harry Menegay
MJR 96-well PTC-1 machine: suitable microtitre plates? Eric H. Mercer
Glycogen Steve Minchin
Tricine for electrophoresis Ding Ming
Need help - black dots on DNA sequencing gels? Robert E Minto
Mitochondrial replication Sean David Moore
what's the best way to clone PCR products? Kevin Morano
Th1 and Th2 separation? Shahram Mori
5 Kb by RT-PCR Shahram Mori
RT-PCR GAP in HL-60 Shahram Mori
Taq Pol and 3' primer mismatch? Shahram Mori
help with 5' race Shahram Mori
Glowing BLUE DNA Gels Shahram Mori
DEPC and PCR? A mystery! Shahram Mori
cDNA Challenge************* Shahram Mori
5' RACE Shahram Mori
Type 52 Polaroid films? Who?****** Shahram Mori
Need A journal's Address!!****** Shahram Mori
Plasmid preps for ABI 373A Mike Morris
Dinucleotide Repeat PCR Mike Morris
35S-Met lifetime? Mike Morris
Genomic DNA from fish blood Hilary Morrison
Spontaneous Spectinomycin resistance ? Peter M. Muriana
Colony lifts - detection with biotinylated probes - background? Peter M. Muriana
Membrane protein fusion vectors? Know of any? Peter M. Muriana
calibrating gilsons Peter M. Muriana
Oligo probe to Coding/non-coding strand - is there a preference? Peter M. Muriana
Extraction of DNA from agarose gels T. J. Murphy
Extraction of DNA from agarose gels T. J. Murphy
calibrating gilsons Bernard Murray
CIP or BAP Bernard Murray
Microtiter Plates Bernard Murray
Liver RNA Prep Needed Ola Myklebost
pRIT5 JOHN NEILL
Electroporation Improvements John Nash
DIG labeled probes John Nash
help with 5' race Peter J. Neame
5' RACE Peter J. Neame
TBE precipitation! Richard Near
Isolation protocol for nucleosome and splice-some Chia Jin Ngee
Ivitrogen Electroporator II? Brad Nicholson
Spontaneous Spectinomycin resistance ? Jarmo Niemi
TRACTOR, a T-track location program Jarmo Niemi
TRACTOR, a T-track location program Jarmo Niemi
transfection of T-cells/Retrovirus Garry Nolan
Formaldehyde Inactivation John Nolan
Microwave restriction digests Prenom Nom
test... Prenom Nom
Microwave restriction digests Prenom Nom
T7 RNA Polymerase Chris Noren
RNA freezing Desrochers Normand
transfections Dr. Pamela Norton
Subcloning PCR Andy Law (Big Nose)
CIP or BAP Andy Law (Big Nose)
Subcloning dirty PCR products Andy Law Big Nose
Santa Cruz Biotech. antibodies Stephen Nurrish
Electroporation optimal DNA conc.? OVan-Ham
DNA bending prediction software Jacques Oberto
Recombinant ENA proteins Jaana Ojala
Tnt Sysetem could not work,help. Shuichi Oka
Subcloning PCR Guilherme Co. Oliveira
RPA Assay kits Guilherme Co. Oliveira
PCR directly from phage Guilherme Co. Oliveira
program for Motif identification? Guilherme Co. Oliveira
TK promoter in transgenic mice: expression pattern? No One
PCR from intact bacterial cells Bjorn Orvar
Lacking signal-linearity from the DIG DNA labelling kit Harald Osmundsen
PCR Primers for Charon 4a David L. Osterbur
pGEM3 Accession Number Francis Ouellette
Entrez Francis Ouellette
DNA EXTRACTION FROM PARAFIN Jim Owens
2-D protein gels Jim Owens
Radio-labelling whole cells (bacteria) protocol?? PRAMATHESH PATEL
membrane proteins PHELPS at SASK.USASK.CA
What's the best mini-prep? RICHARD P. PHIPPS
PCR for generating radiolabeled probes? RICHARD P. PHIPPS
LA PCR POPOVA
LA PCR POPOVA
cloning problems POPOVA
PCR from cDNA library POPOVA
ANTIBIOTIC STORAGE POPOVA
pBR322 origin PTJ=Willemsen%omb.acta%preklin at Asklepios.med.vu.nl
Ecoli(ori) PTJ=Willemsen%omb.acta%preklin at Asklepios.med.vu.nl
RACE Help me please!! Paiks
Serum protein after repeated freeze-thaw Paiks
Problems with 1.5 ml Eppendorf tubes Anthony Palombella
Entrez Julian Parkhill
RPA ASSAY Aaron Pawlyk
Dinucleotide Repeat PCR Peter
Blood DNA (Pierre C.)- Peter
MagnaSphere paramagnetic particles (SERVA) Peter
Non radioactive Northern Blot Procedure James N Petitte
Methods for Ascorbate Phosphatase Thanit Pewnim
Assay of Ascorbate Sulfatase Thanit Pewnim
References on the use of SHRIMP alkaline phosphatase Thanit Pewnim
E. coli origin PhJ=van=Dalen%omb.acta%preklin at Asklepios.med.vu.nl
raising ANTIBODIES with fusion proteins?? T. S. Pillay
homemade receipt for ECL reagents T. S. Pillay
PC12 cells- how to culture T. S. Pillay
Database for tyrosine phosphorylated proteins? T. S. Pillay
STOP ADVERTISING !!! T. S. Pillay
PC12 cells- how to culture Michael Poidinger
what's the best way to clone PCR products? Michael Poidinger
SEQUENCING OF PCR PRODUCTS?? HMMMMMMMMMMM Michael Poidinger
what's the best way to clone PCR products? Michael Poidinger
DNA sequencer Michael Poidinger
ABI sequencers with Genpak seq kits Richard T. Pon
Methods for the measurement of bilirubin Michael Portmann
microbial activity in soil Dick Pratt
what's the best way to clone PCR products? Mike Preston
black dots sequencing gels Robert Preston
MW Markers for RNA gels... M.J. Principe
PCR Clean up, 150bp frag??? Kevin Pumiglia
Searching for silent mutations... Kevin Pumiglia
GST fusion: Solution and more questions Kevin Pumiglia
Clostridium acetobutylicum cultures Rod Putland
antisense oligo questions GLEN RAFFEL
35 mm slides of gels - help RAMPITSCH at BCRSSU.AGR.CA
HELP-- TRANSFECTION GRADE PLASMID DNA RMARTINQFD at BIOTECHNET.COM
inducible vectors ROSS at SCI.WFEB.EDU
gastropod mucus Adel Rajab
Ivitrogen Electroporator II? Uttam Rajbhandary
Air cyclers and sequencing Randy P. Rasmussen
Tnt Sysetem could not work,help. Marko Rehn
looking for Xenopus cDNA libraries Tanya Reichert
Hirt prep Mary Remington
MW Markers for RNA gels... Douglas Rhoads
Fast thermocycler Douglas Rhoads
radiation counters Douglas Rhoads
graphics from GCG programs Peter Rice
Perkin Elmer VS Thermolyne: Recommendations? RichWalko
need help in analysing fatty acidsmenu Richard
Where to get ppGpp?? Richard
tritium-labelled proteins: best detection method Richard
Determination of genome size (Cot curves) RiverSloth
vector lab. fax Monique Rogard
>Degas per min & Ultrasound? Dr. A. Rosenthal
membrane proteins RoundUp
Immunoaffinity Columns RoundUp
black dots on DNA sequencing gels? Joan Rowe
artifact bands on sequencing gels Joan Rowe
SEQUENCING OF PCR PRODUCTS?? HMMMMMMMMMMM Joan Rowe
Fuji film sensitivity loss Joan Rowe
RT-PCR GAP in HL-60 Janet E. Rubin
TA cloning doesn't work if a certain base is at 5' end of primer? Robert Rumpf
TA cloning doesn't work if a certain base is at 5' end o Robert Rumpf
TA cloning doesn't work if a certain base is at 5' end o Robert Rumpf
Rain-X and sequencing plates Peter J. Russell
pCp (NOT the drug) SCHNAUFER at ibc.unibe.ch
DNA from agarose gels GLEN SHEARER
MW Markers for RNA gels... SPIKE
Macintosh Software for teaching Genetics SPIKE
PCR SEQUENCING ADVICE Edgar Salazar-Grueso
expression/induction depends of container Roland J Saldanha
Oligo synthesisers: Millipore Expedite vs Beckman Oligo 1000 Roland J Saldanha
Phosphoroimagers vs Instantimager Experiences Saught Roland J Saldanha
Non radioactive Northern Blot Procedure Klaus Salger
Extraction of DNA from agarose gels Klaus Salger
small DNA fragment recovery Klaus Salger
Plasmid preps for ABI 373A Lukasz Salwinski
takara address Rebecca Schall
Apotag Kit (cell death kit) Rebecca Schall
Re-subscribe Steve Schramm
Northerns Alan H. Schulman
Needed: Human CNS cDNA library Paul Scotting
RNA isolation from small # of cells? Paul Scotting
T-tailing for PCR product ligation Loryn Sellner
PCR primers for TCR Loryn Sellner
Blue/white colony selection problem. G Selvarani
T-tailing for PCR product ligation G Selvarani
Nonisotopic AA transport assays (cont.) Hr Dr. S. Shapiro
Nonisotopic AA transport assays: summary Hr Dr. S. Shapiro
Source of multi-filtre manifolds Hr Dr. S. Shapiro
Mitochondrial genome size determination Raman Sharma
T-tailing for PCR product ligation J Shepherd
expression libraries J Shepherd
Luciferase Assay Yoshiyuki Shibata
PCR without protein extraction? Mark Siddall
Help...can't stop RBC lysis in trout blood Mark Siddall
Taq left at RT - gulp! - Mark Siddall
pGEM3 Accession Number Simmons, Alice 2562
are there rat specific primers available Henry Simpkins
Taq Pol and 3' primer mismatch? Rob Slebos
Racks for Sterilin Universals? Brian Smith
RFD: sci.bio.microbiology Una Smith
RPA Assay kits Rick Smoke
RPA ASSAY Rick Smoke
Recipe for linear polyacrylamide Mark Solomon
Promega Cell Titer Kit Janet De Souza
GST fusion: Solution and more questions Pavel Sova
Inexpensive blotting paper? Anne Spang
raising ANTIBODIES with fusion proteins?? Anne Spang
Recommendations for light level protein detection? Anne Spang
Epitope-Tagging and EM Anne Spang
Epitope-Tagging and EM Anne Spang
Promega on the Internet Stephan Spencer
Looking for mol. biol. courseware Edwin van Spronsen
Trizol reagent problems Murali Srinivasan
ABI sequencing artifact Murali Srinivasan
Other Lactose analogs Jamie Stanek
Methods for the measurement of bilirubin StanleyDSF
COMPETENT CELLS PROTOCOL!!! Darren Stauth
Northern blots Ed Stephenson
small DNA fragment recovery David B. Stern
Project Leader in Cell Culture Oh Kah Weng Steve
RNA tips B Stickney
sephadex G-50 B Stickney
RNA Probe woes B Stickney
Leader Peptides/Attenuators Matthew Stoecker
Rhodococcus shuttle vector Matthew Stoecker
ABI sequencing artifact Matthew Stoecker
graphics from GCG programs Marty Stoltzfus
graphics from GCG programs Marty Stoltzfus
Columns for miniprep DNA Marty Stoltzfus
NSF Postdocs Joan Strassmann
Cell cycle analysis kits Michael Strehle
embryonic stem cells David Strehlow
Help-need clipart of DNA molecule Gregory Stuart
takara address SAtyam Subrahmanyam
(none) Zalman L. Suldan
Microtiter Plates Zalman L. Suldan
Aff. purified. anti hVitronectin. Jan Svensson
RNA isolation Agnieszka Szczepek
method(s) to measure ATP NICHOLAS THEODORAKIS
RNA tips NICHOLAS THEODORAKIS
G50 to remove unicorporated isotope NICHOLAS THEODORAKIS
Transients-DMSO shock. NICHOLAS THEODORAKIS
Where to get shrimp alk. phosphatase? NICHOLAS THEODORAKIS
35S-Met lifetime? NICHOLAS THEODORAKIS
Reticulocyte Lysate NICHOLAS THEODORAKIS
T7 Expression System Song Tan
gel retardation assay Song Tan
Need help - black dots on DNA sequencing gels? Song Tan
Pefabloc (Was: membrane proteins) Song Tan
Promega on the Internet Song Tan
Promega on the Internet Song Tan
Air Thermocycler and 32P? Amy L Teel
LA PCR Nancy Templeton
Linear polyacrylamide as an alternative to glycogen carrier Dietmar Tietz
Making buffer Anthony Tomlinson
Electroporation Improvements Anthony Tomlinson
SEQUENCING OF PCR PRODUCTS?? HMMMMMMMMMMM Anthony Tomlinson
Cre/lox vectors Anthony Tomlinson
Beware pGEM-T Anthony Tomlinson
DNA-affinity columns Joanne Tornow
Oligo synthesisers: Millipore Expedite vs Beckman Oligo 1000 Toromtomtom
PCR subcloning Mel Trecker
MJR 96-well PTC-1 machine: suitable microtitre plates? Martin Trick
Sources for modified nucleotide triphosphates Rick Tullis
Seeking USED Cryostat Bradley Turner
Perkin Elmer VS Thermolyne: Recommendations? U58563 at uicvm.uic.edu
calibrating gilsons U58563 at uicvm.uic.edu
pcr Juan C. Mendible UCV
PCR and HPV Juan C. Mendible UCV
lambda vectors without BglII/MunI sites Peter Uetz
MW Markers for RNA gels... Paul E. Ulanch
PCR Product Purity Assay VICDEF at BIOLOGY.BIOSCI.WAYNE.EDU
protein fluorescence: NaI quenching??? VOSS at AIMP1.UNA.AC.AT
pipette tune-ups Paul A. Vescio
pipette tune-ups Paul A. Vescio
microbial activity in soil Paul A. Vescio
PVP, Microwavable? Paul A. Vescio
Help-need clipart of DNA molecule Paul A. Vescio
T-tailing for PCR product ligation Paul A. Vescio
16S RNA E-mail server? Paul A. Vescio
16S RNA E-mail server? Paul A. Vescio
Colony lifts - detection with biotinylated probes - background? Paul A. Vescio
Amplification of plasmids NOT with Chloramphenicol I.B. Vipond
Reticulocyte Lysate Sam Volchenboum
Reticulocyte Lysate Sam Volchenboum
Help needed with transfection of Jurkat Dominic Voon
.01um pore filters Karl Voss
DNA-binding protein WANZ at QUCDN.QueensU.CA
gel retardation WANZ at QUCDN.QueensU.CA
gel retardation WANZ at QUCDN.QueensU.CA
protein phosphorylation leading to DNA binding WANZ at QUCDN.QueensU.CA
Promega on the Internet WAS
Looking for a very tight binding Ab/epitope pair WEDEL at vms.biochem.mpg.de
Ribosomal RNA prep? Michael van Waes
Where to get shrimp alk. phosphatase? Michael van Waes
5'->3' InhibitACE Michael van Waes
Black dots on Sequence Films James M Wahlberg
16S RNA E-mail server? James M Wahlberg
Internal ribosome binding sites. Warren Wakarchuk
human cosmid library? Wallner, Martin
Buying Hyb. Oven/Roller Shiao Y. Wang
Inexpensive blotting paper? Shiao Y. Wang
PCR: Promega Taq vs. Perkin-Elmer Taq Shiao Y. Wang
Fast thermocycler Shiao Y. Wang
PCR SEQUENCING ADVICE Shiao Y. Wang
Blood DNA Shiao Y. Wang
Rain-X and sequencing plates Shiao Y. Wang
PCR w/ capillary tubes Shiao Y. Wang
Ultra-low Freezers Shiao Y. Wang
Fast thermocycler Shiao Y. Wang
35 mm slides of gels - help Shiao Y. Wang
PCR PRIMER DESIGN HELP!! Wenfu Wang
Adress for Dr. Moshe Oren, Weizmann Ins Pearse Ward
Sequencing using infrared tags Lawrence Washington
modified oligos Lawrence Washington
Li-cor DNA-Sequencer Lawrence Washington
Primers with dI vs mixed bases? John Watson
PCR: Promega Taq vs. Perkin-Elmer Taq John Watson
pcDNA1amp sequence John Watson
human genomic DNA PCR John Watson
vancomycin and Agrobacterium Andreas Weber
Promega on the Internet Christoph Weber
Promega on the Internet Helge Weissig
In situ question Dan Wetzel
PCR: Promega Taq vs. Perkin-Elmer Taq Ross Whetten
PCR product cloning with DK101 vector Dr. R.B. White
Deletion Factory System (Gibco) Steve Whitwill
BRL Deletion Factory System Steve Whitwill
SEQUENCING OF PCR PRODUCTS?? HMMMMMMMMMMM Roger Wiegand
fluorescent primers for DDRT-PCR Roger Wiegand
Where to get shrimp alk. phosphatase? Roger Wiegand
calibrating gilsons Wiggy
Method for Touchdown PCR Wiggy
genetics research Leah Williams
PCR from intact bacterial cells Troels Wind
Phage display expression library Troels Wind
Schneider-2 M3 medium Neil Winegarden
cytosine deaminase, commercial source? Larry Winger
cytosine deaminase sources, please Larry Winger
NIH netters help with ZAP Linda Wolff
Monoclonal Ab to Chlamydia Surasakdi Wongratanacheewin
Anyone using Pharmacias EasyPrep device? Jim Woodgett
Are Ab's phosphorylated? Jim Woodgett
Know of a Penicillium Library? X-Man
expression/induction depends of container XRDPROC at Gems.VCU.EDU
RNA isolation failure Lin Xiao
homemade receipt for ECL reagents OKADA Yasushi
Taq left at RT - gulp! - Ann M. Yezerski
@#! smears on PCR ??? Ann M. Yezerski
pcDNA1amp sequence Ian A. York
raising ANTIBODIES with fusion proteins?? Ian A. York
CIP or BAP Ian A. York
PCR Clean up, 150bp frag??? Ian A. York
Electroporation of COS: How to? Ian A. York
Problems with 1.5 ml Eppendorf tubes Lester Young
wanted: 2 umole scale synthesis of ribo-oligo (4mer) for under $150 Dongxian Yue
Dinucleotide Repeat PCR Bob Zahorchak
PCR: Promega Taq vs. Perkin-Elmer Taq Zainul Zain
Sephadex G-50 Zainul Zain
what's the best way to clone PCR products? Angel Zarain
what's the best way to clone PCR products? Angel Zarain
Nuclear extracts Matthias Zeiner
two hybrid system and differential display Christie Doris Zerylnick
Problems with cDNA-synthesis Mattias Zetterstrand
gel pull off Dmitry Zharkov
Good mRNA Isolation Kit Xin-Hua Zhou
faint bands in PCR Xin-Hua Zhou
Linear polyacrylamide as an alternative to glycogen carrier Frances Hannan Zoology
DIG labeling PCR probes II Frances Hannan Zoology
Rain-X Frances Hannan Zoology
Polypeptides ae55
How to transform CRL-1999? aeschba at fmi.ch
Seq.power supply David Micklem alt.ascii-art alt.ascii-art.animation alt.asian-movies alt.astrology alt.atari-jaguar.discussion alt.atari.2600 alt.atheism alt.atheism.moderated alt.atheism.satire alt.ato alt.autos.antique alt.autos.camaro.firebird alt.autos.karting alt
Promega Taq vs. Perkin-Elmer Taq an125249 at anon.penet.fi
DNA sequencer b137795 at mailserv.cuhk.hk
(none) babalola at MAIL.SAS.UPENN.EDU
DD/PCR products hang on wells babalola at MAIL.SAS.UPENN.EDU
Re-diff. display problems babalola at MAIL.SAS.UPENN.EDU
T-tailing for PCR product ligation being
Dinucleotide Repeat PCR berschic at convex.rz.uni-duesseldorf.de
gel retardation assay shantaram bharadwaj
Non radioactive Northern Blot Procedure books at mcvax2.d48.lilly.com
Non radioactive Northern Blot Procedure books at mcvax4.d48.lilly.com
semliki forest virus brett at BORCIM.WUSTL.EDU
T4 lysozyme lesley brown
T4 Lysozyme summary lesley brown
tryptophan synth and hydroquinone brwgor01 at uctvax.uct.ac.za
Affinity column - for a-amylase camsubbu at ubvms.cc.buffalo.edu
Concealed clontech!CHRISC at WPSMTP.netcom.com
Who has this stuff? clontech!CHRISC at WPSMTP.netcom.com
(none) clontech!pauld at NETCOM.COM
<None> cyto at cc.usu.edu
test question/problem solving cyto at cc.usu.edu
tritium-labelled proteins: best detection method dacoj3 at wptemp.kvl.dk
Looking for Yeast Genetic Stock art dart
Looking for Yeast Genetic Stock art dart
Pisia vs Baculovirus, opinions wanted art dart
SYBR Green I dhanzel at delphi.com
Re-using SYBR Green? dhanzel at delphi.com
ABI sequencing artifact dina at libpc1.icos.com
DNA from AGPC Preps dkim at unm.edu
RNA Precipitation with Ammonium Acetate dkim at unm.edu
help with protein expression eb86336 at inet.d48.lilly.com
faint bands in PCR eb86336 at inet.d48.lilly.com
Blue/white colony selection problems eb86336 at inet.d48.lilly.com
columns for miniprep dna eb86336 at inet.d48.lilly.com
Patenting of Biotechnology eb86336 at inet.d48.lilly.com
Subcloning PCR eb86336 at inet.d48.lilly.com
Internal RBS. eb86336 at inet.d48.lilly.com
Ligating small fragments into large vectors. eb86336 at inet.d48.lilly.com
HCHO reactivity euverink at biol.rug.nl
Tightly regulated E. coli Promoters ez001427 at dale.ucdavis.edu
5' filling with Klenow ez003450 at chip.ucdavis.edu
inactivation of phosphatase ez003450 at chip.ucdavis.edu
Blue sectors from plasmids with inserts ez003450 at chip.ucdavis.edu
DR III, PFGE falkj-bisance
Glowing Blue Spots fnord
Glowing BLUE DNA Gels roy french
Restriction map of EMBL4 wanted. futers at biovax.leeds.ac.uk
Extraction of DNA from agarose gels futers at biovax.leeds.ac.uk
Fuji film sensitivity loss g_watson at icrf.icnet.uk
Fuji Film Loss of Sensitivity g_watson at icrf.icnet.uk
Electroporation of COS: How to? g_watson at icrf.icnet.uk
use of BSA in EMSA supershifts gc
Glycogen gc
Yeast Nitrogen Base?? gibbs
Any better way to get rid of MDE gel ? gli at UCDAVIS.EDU
Tricine for electrophoresis grflentk at macc.wisc.edu
use of BSA in EMSA supershifts grgmj at picr.cr.man.ac.uk
Retard gel buffering conditions grgmj at picr.cr.man.ac.uk
expression/induction depends on container hardies at BALIN.UTHSCSA.EDU
cloning problems hl15 at hubrecht.nl
Culturing B lymphocytes immdj at picr.cr.man.ac.uk
Single Copy number plasmids j.m.lodge at bham.ac.uk
zymograms for detecting lipase activity jd89
RBC lysis in trout blood jd89
Pisia vs Baculovirus, opinions wanted jfh
human genomic DNA PCR jfh
what's the best way to clone PCR products? jfh
Reverse Northern Blot? jfh
Oncor's Apoptag--comments, users? jfh
glycoprotein isolation jgill at kean.ucs.mun.ca
Measuring Intracellular Calcium Concentration jobersta at
DNA Polymerase "extendase" activity jobling at usuhs.usuhs.mil
DNA EXTRACTION FROM PARAFIN john
Does anyone have experience with LONG PCR? kamin johnson
TBE precipitation! jr at dna.bio.warwick.ac.uk
centrifugal vacume concentraters jurgenson at cobra.uni.edu
Kohara mapping membrane kertesz at micro.biol.ethz.ch
non-radioactive cycle sequencing klingj
test kofoid
Need help - black dots on DNA sequencing gels? kofoid
Fast thermocycler kofoid
Problem with inversed binding on a dot blot kramerse at wmvx.lvs.dupont.com
NORTHERN BLOTS kvernick at helix.nih.gov
PCR Clean up, 150bp frag??? kvernick at helix.nih.gov
PCR for radiolabelling probes kvernick at helix.nih.gov
pGEM accession numbers lidaniel
PCR with 3' mismatch m-brazil at nimr.mrc.ac.uk
t-tailing vector of choice m-brazil at nimr.mrc.ac.uk
RNA tips (DEPC and nucleic acid) madani at vms.cis.pitt.edu
Ultra-low Freezers john markwell
CIP or BAP matthews
pcDNA3 mbjpl at seqnet.dl.ac.uk
two hybrid system and differential mccallo at rand
PCR: Promega Taq vs. Perkin-Elmer Taq merlin
Curiosity about Biotechnology/Molecular Biology Patents merlin
10/17/94 - US Patent Office Hearing on Biotechnology - San Diego, CA merlin
STOP ADVERTISING !!! merlin
ADVERTISING IN BIONET merlin
William D. Huse / Ixsys, Inc -- San Diego, CA merlin
Looking for mol bio software.... micha at amber.biophys.uni-duesseldorf.de
graphics from GCG programs micha at amber.biophys.uni-duesseldorf.de
about filber digestive GENE(s) mluo at cc.memphis.edu
method(s) to measure rATP mmccarthy at delphi.com
Plasmid prep from dead agar plates mrege at samba.cnb.uam.es
Seeking rfa, rfb clones mrege at samba.cnb.uam.es
subcloning PCR mthomson at bscr.uga.edu
how does streptavidin bind biotin? murali at unixg.ubc.ca
RPA Assay kits nishir at ohsu.edu
Carlisle Landel! nishir at ohsu.edu
PC12 cells- how to culture nishir at ohsu.edu
Problems with cDNA-synthesis nishir at ohsu.edu
what's the best way to clone PCR products? nishir at ohsu.edu
sequencing nussbaum at neurochem.u-strasbg.fr
sequencing nussbaum at neurochem.u-strasbg.fr
glycoprotein purification nussbaum at neurochem.u-strasbg.fr
TGF beta related ott at wmvx.lvs.dupont.com
RAPD sequencing palasj9579 at cobra.uni.edu
PCR from cDNA library parin
RC20 anti-phosphotyrosine Ab: non-specific detection partis
Cycle sequencing - no product partis
Cyclophilin (prolyl isomerase) suppliers? pathology at mscf.med.upenn.edu
secondary screening after differential display pattonaj%phvax.dnet at sb.com
Streptomyces PCR mutagenesis pdxrmb at vaxa.nott.ac.uk
measuring mice blood pressure carlos h. pedemonte
measuring TX/mice blood pressure carlos h. pedemonte
5'RACE & methyl-G mRNA cap pmiguel at bilbo.bio.purdue.edu
Epitope-Tagging and EM wang qi
Rapp Polymere resin quarrell at vax.oxford.ac.uk
where to get Rapp Polymere TentaGel? quarrell at vax.oxford.ac.uk
Where to get TG-1 and TG-2 qyuan at gnv.ifas.ufl.edu
2-naphthoyltrifluoroacetate r
Automated Hybridisation r_alderton at icrf.icnet.uk
Automated Hybridisation r_alderton at icrf.icnet.uk
PCR from intact bacterial cells kenneth garson cancer research
Air cyclers and sequencing doug rhoads
Fast thermocycler doug rhoads
Where is the sequence of pRE4??? rosas at mcclb0.med.nyu.edu
Sequence of the shuttle vector pRE4 rosas at mcclb0.med.nyu.edu
Restriction map of EMBL4 wanted. rr at umesci.maine.edu
SEQUENCING OF PCR PRODUCTS?? HMMMMMMMMMMM russos at HAL.HAHNEMANN.EDU
Probe Source? rvosatka at opal.tufts.edu
ZAP Express? rvosatka at opal.tufts.edu
Looking for Renex-30 sandra at physio.uct.ac.za
high luciferase background schupp at nauvax.ucc.nau.edu
MJR 96-well PTC-1 machine: suitable microtitre plates? schupp at nauvax.ucc.nau.edu
microwave RE digests in microtiter plates? schupp at nauvax.ucc.nau.edu
Yeast Nitrogen Base?? warren shore
5' RACE swift at mcvax4.d48.lilly.com
RNA isolation from small # of cells? szcooley at chip.ucdavis.edu
lambda/HindIII szcooley at chip.ucdavis.edu
Dinucleotide Repeat PCR szcooley at chip.ucdavis.edu
black dots on DNA sequencing gels? szcooley at chip.ucdavis.edu
black dots sequencing gels szcooley at chip.ucdavis.edu
Columns for miniprep DNA szcooley at chip.ucdavis.edu
Electroporation Improvements szcooley at chip.ucdavis.edu
TBE precipitation! szcooley at chip.ucdavis.edu
T-tailing for PCR product ligation szcooley at chip.ucdavis.edu
Reverse Northern Blot? szcooley at chip.ucdavis.edu
Method for Touchdown PCR szcooley at dale.ucdavis.edu
TA cloning and making your own T-vector szcooley at dale.ucdavis.edu
Ta cloning and making your own T-vector szcooley at dale.ucdavis.edu
Secondary screening after differential display? szcooley at dale.ucdavis.edu
help with protein expression szjmahr at dale.ucdavis.edu
Schneider-2 M3 medium tahilyamani.com at syntex
method(s) to measure rATP tjames at eagle.wesleyan.edu
pGEM accession numbers tronni at ktl.fi
TA cloning and making your own T-vector txpljfg at UABCVSR.CVSR.UAB.EDU
Ta cloning and making your own T-vector txpljfg at UABCVSR.CVSR.UAB.EDU
black dots. vdrover at kean.ucs.mun.ca
sephadex G-50 vdrover at kean.ucs.mun.ca
deprobing problem vdrover at kean.ucs.mun.ca
Rain-X vdrover at kean.ucs.mun.ca
ribosomal RNA prep vdrover at kean.ucs.mun.ca
Help...can't stop RBC lysis in trout blood vdrover at kean.ucs.mun.ca
RNA tips (DEPC and nucleic acid) michele young
Catrimox.14 michele young

Last message date: Fri Sep 30 23:03:22 EST 1994
Archived on: Sun Mar 6 20:15:28 EST 2005

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