Methods September 1994 Archives by thread
Starting: Thu Sep 1 00:35:05 EST 1994
Ending: Fri Sep 30 23:03:22 EST 1994
Messages: 1197
- Tnt Sysetem could not work,help.
Shuichi Oka
- More Densitometry software?
Mr TG Downing
- Sources for modified nucleotide triphosphates
Rick Tullis
- where is Cambridge Res. Biochem.?
daj (David Johnston)
- where is Cambridge Res. Biochem.?
B.G.Macdonald
- PCR from intact bacterial cells
Troels Wind
- glycoprotein isolation
jgill at kean.ucs.mun.ca
- vancomycin and Agrobacterium
Andreas Weber
- Needed: Human CNS cDNA library
Paul Scotting
- Inexpensive blotting paper?
Anne Spang
- Microwave Miniprep - protocol?
Mr TG Downing
- Methods for the measurement of bilirubin
Michael Portmann
- RNA isolation from small # of cells?
Paul Scotting
- pGEM accession numbers
lidaniel
- Hirt prep
Mary Remington
- Taq DNA Pol and frameshifts
Hanson Lab
- blunt end ligation
Ryszard Kole
- RT-PCR
Ryszard Kole
- Is downward transfer REALLY good for Northern?
Tracy Aquilla
- primers with dI vs mixed bases
Emma Macfarlane
- Blue/white colony selection problem.
Wises Namwat - SCMI - 3636183
- T7 Expression System
Song Tan
- e.coli transformation question
the End
- Spontaneous Spectinomycin resistance ?
the End
- Clontech's Transformer Kit- Help please
Dave Boyd
- PCR with 3' mismatch
Curt Ashendel
- site directed mutagenesis
Tracy Aquilla
- Sequencing Gel Spacer Plastic
Tracy Aquilla
- Promoter and DNA consensus-finding software
Tracy Aquilla
- NORTHERN BLOTS
Tracy Aquilla
- mRNA storage
Tracy Aquilla
- Laser densitometers
Jack M. Bernstein, M.D.
- help with protein expression
szjmahr at dale.ucdavis.edu
- rescue dead stock
Paul N Hengen
- Buying Hyb. Oven/Roller
Tracy Aquilla
- PGEM accession numbers
Tracy Aquilla
- DATA MANAGEMENT of ABI373 Core Sequencing Facility
Richard R. Hardy
- membrane proteins
PHELPS at SASK.USASK.CA
- SYBR Green I, more exper
Jeanne Loring
- Lost DNA after precipitation
David L. Haviland, Ph.D.
- T7 Expression System
Tracy Aquilla
- graphics from GCG programs
Marty Stoltzfus
- NORTHERN BLOTS
David L. Haviland, Ph.D.
- MW Markers for RNA gels...
SPIKE
- RNA isolation from small # of cells?
szcooley at chip.ucdavis.edu
- Looking for mol bio software....
Tracy Aquilla
- Plasmid preps for ABI 373A
Lisa Bibbs
- embryonic stem cells
David Strehlow
- pGEM accession numbers
tronni at ktl.fi
- pGEM3 Accession Number
Simmons, Alice 2562
- Bacterial Chromosomes and PFGE
Brian_D Lanoil
- Subcloning PCR
Jeanne Loring
- Any experience with Soccorex pipettors
John H McDonald
- pipette tune-ups
John Korte
- DNA Polymerase "extendase" activity
Enrique Jose Labadan Frio - SCBC - 3421101
- PCR: Promega Taq vs. Perkin-Elmer Taq
merlin
- Subcloning dirty PCR products
Andy Law Big Nose
- sequencing
nussbaum at neurochem.u-strasbg.fr
- CDM8 sequence
Georges Gaudriault
- Inexpensive blotting paper?
B.L.Cohen
- MW Markers for RNA gels...
Douglas Rhoads
- Other 'merlin' celite preps out there?
Charlie Wright Genetics
- DNA cruciforms and Mg2+ in vivo ?
Horst Ibelgaufts
- sequencing
nussbaum at neurochem.u-strasbg.fr
- Secretion helper vector for E.coli
Priyal A. De Zoysa now at Royal Free
- simian virus 40 we are looking for a plasmid map of pSV-7 as discribed by Colby and Shenk in 1982 (PNAS)
at uni-muenster.de
- RT activity in Taq pol?
Tracy Aquilla
- lambda/HindIII
szcooley at chip.ucdavis.edu
- Fusion protein was trapped in the cell pellet
S.A. Benson Lab
- Fusion protein was trapped in the cell pellet
S.A. Benson Lab
- Protein electroelution from SDS-page
JoeJoe
- Plasmid preps for ABI 373A
Ghis Allard
- IMPORTANT! - BIOSCI at net.bio.net off-line Saturday PM.
BIOSCI Administrator
- RT in taq
NEAL MUSTO
- list of E. coli genotypes
HARDIES at THORIN.UTHSCSA.EDU
- amino acid frequency
Shaun D. Black
- zymograms for detecting lipase activity
jd89
- Leader Peptides/Attenuators
Matthew Stoecker
- Sequencing using infrared tags
Peter Gegenheimer
- RT activity in Taq pol?
Klaus.Matthaei at ANU.EDU.AU
- embryonic stem cells
Klaus.Matthaei at ANU.EDU.AU
- lambda/HindIII
John Brunstein
- DNA Polymerase "extendase" activity
jobling at usuhs.usuhs.mil
- Phage DNA storage temp.
Joshua Daniels
- HELP-- TRANSFECTION GRADE PLASMID DNA
RMARTINQFD at BIOTECHNET.COM
- Rapp Polymere resin
quarrell at vax.oxford.ac.uk
- Rhodococcus shuttle vector
Matthew Stoecker
- Catalogs for microscopy?
Jun Kim
- transfection of T-cells/Retrovirus
Garry Nolan
- Curiosity about Biotechnology/Molecular Biology Patents
merlin
- teaching molecular biology lab to undergrad students
Prasit Palitpolkanpim - GRSO
- (none)
Laura Anne Bannister
- gel retardation assay
shantaram bharadwaj
- gel retardation assay
Song Tan
- RFD: sci.bio.microbiology
Una Smith
- PEST-FIND algorithm
Darrick Carter
- Good mRNA Isolation Kit
Xin-Hua Zhou
- Th1 and Th2 separation?
Shahram Mori
- Buying Hyb. Oven/Roller
Shiao Y. Wang
- Oligo dT
Too Heng-Phon
- Problem getting JM110 competent
Duncan Clark
- testing
Dorothea.Smith
- microbial activity in soil
Dorothea.Smith
- Looking for A Company; Australia.
B.G.Macdonald
- re: PCR from intact bacterial cells
CORROCHANO PELAEZ, LUIS M.
- Why is tet-repressor system on two plasmids?
Graham Atherton
- Nonisotopic AA transport assays (cont.)
Hr Dr. S. Shapiro
- Automated Hybridisation
r_alderton at icrf.icnet.uk
- Dinucleotide Repeat PCR
LICHY at EMAIL.AFIP.OSD.MIL
- Effect of PEG on rxns
HARDIES at THORIN.UTHSCSA.EDU
- Gel Supershifts
Dominique Marilley
- Linear Plasmid
Ali Karami
- PEG debate
JAB5 at VAX.YORK.AC.UK
- where is Cambridge Res. Biochem.?
John E. Fox
- Help wanted, RNA isolation
Jackie Fetherston
- Effect of PEG on rxns
Ashok Aiyar
- PCR from intact bacterial cells
Bjorn Orvar
- genetics research
Leah Williams
- Need restriction map of pT7.7
CBA0034 at uabdpo.dpo.uab.edu
- COMT reaction using erythrocytes
Ilonka Guenther
- PVP, Microwavable?
Paul A. Vescio
- genetics research
Wolfram Brune
- Oligo dT
Olav Hungnes
- Dinucleotide Repeat PCR
berschic at convex.rz.uni-duesseldorf.de
- Efficient transfection into HepG2
Yoshiki Kawabe
- ligation
R Hanemaaijer
- Need help - black dots on DNA sequencing gels?
Robert E Minto
- ligating small fragments into large vectors
Jack Jenuth
- pcDNA1amp sequence
Ian A. York
- PCR inhibitors in insect tissue?
Andrew Cockburn
- black dots on DNA sequencing gels?
Susan E Ingraham
- black dots on DNA sequencing gels?
szcooley at chip.ucdavis.edu
- More Bacterial Chromosomes and PFGE
Brian_D Lanoil
- PCR screening: False positives
Dr. W. Greenhale
- Reuse of Qiagen columns
Lakshmi Madabusi
- graphics from GCG programs
Marty Stoltzfus
- Need help - black dots on DNA sequencing gels?
Song Tan
- shearing DNA to 1kb size
Michael Gorry
- black dots sequencing gels
Robert Preston
- ABI sequencing kit
Tim Buss
- method(s) to measure rATP
tjames at eagle.wesleyan.edu
- (none)
clontech!pauld at NETCOM.COM
- test
kofoid
- raising ANTIBODIES with fusion proteins??
Ian A. York
- Disappearing Clones during Library Screening
Jeff Dean
- raising ANTIBODIES with fusion proteins??
Anne Spang
- black dots on DNA seq. gels
HARDIES at THORIN.UTHSCSA.EDU
- Looking for pGK12 plasmid or creators
Derek Jones
- Santa Cruz Biotech. antibodies
Stephen Nurrish
- (none)
babalola at MAIL.SAS.UPENN.EDU
- DD/PCR products hang on wells
babalola at MAIL.SAS.UPENN.EDU
- gel pull off
Dmitry Zharkov
- Where to get TG-1 and TG-2
qyuan at gnv.ifas.ufl.edu
- raising ANTIBODIES with fusion proteins??
Riccardo Fodde
- Yeast expression library ??
Darrick Carter
- NORTHERN BLOTS
kvernick at helix.nih.gov
- Help-need clipart of DNA molecule
Gregory Stuart
- Seq Gel Spots
DAVID MITCHELL
- Urgent! Fax-Number from Vical Inc. needed!
Inst. f. Biochemistry
- CAT mRNA
J Grimbergen
- Hexamer Primer Walking Sequencing
Dr. M.D. Jones
- Lacking signal-linearity from the DIG DNA labelling kit
Harald Osmundsen
- Eastern blotting!
Nigel Eastmond
- reusing agarose gels
Nigel Eastmond
- NIH Imaging software
Nigel Eastmond
- transfection of monocytic cells
Markus Guetlich
- transfection of monocytic cells
Markus Guetlich
- TRACTOR, a T-track location program
Jarmo Niemi
- black dots.
vdrover at kean.ucs.mun.ca
- use of BSA in EMSA supershifts
grgmj at picr.cr.man.ac.uk
- What video-camera for DNA-Gels?????
Andreas Langsdorf
- Seq Gel Spots"
DAVID MITCHELL
- transfections
Dr. Pamela Norton
- high luciferase background
schupp at nauvax.ucc.nau.edu
- Internal ribosome binding sites.
Warren Wakarchuk
- looking for nucleosidase
Pierre Commercon
- Primers with dI vs mixed bases?
John Watson
- 5'RACE & methyl-G mRNA cap
pmiguel at bilbo.bio.purdue.edu
- Looking for Yeast Genetic Stock
art dart
- subscribe
Richard Kenneth Bruce Martel
- Other Lactose analogs
Jamie Stanek
- differential display PCR
HARDIES at THORIN.UTHSCSA.EDU
- SSCP help
Frederick Garbrecht
- subscribe
Lisa Bibbs
- to T. Bickle
Rafa Maldonado
- Quantifying mouse IFNAR???
Mary Coussons-Read
- Quantifying mouse IFNAR???
Mary Coussons-Read
- SSCP help
HARDIES at THORIN.UTHSCSA.EDU
- Sephadex G-50
Maureen Bunger
- human cosmid library?
Wallner, Martin
- Glowing Blue Spots
fnord
- Dinucleotide Repeat PCR
Peter
- Double tet expression system
Graham Atherton
- Looking for Yeast Genetic Stock
Priyal A. De Zoysa now at Royal Free
- RC20 anti-phosphotyrosine Ab: non-specific detection
partis
- 'Easy' Blue/white cloning
Duncan Clark
- sephadex G-50
vdrover at kean.ucs.mun.ca
- pBR322 origin
PTJ=Willemsen%omb.acta%preklin at Asklepios.med.vu.nl
- inducible vectors
ROSS at SCI.WFEB.EDU
- Plasmid preps for ABI 373A
Ghis Allard
- direct transfection of PCR products
Seth Daniel Crosby
- needed Cell/Molecular Biologist
Seth Daniel Crosby
- PCR from intact bacterial cells
Seth Daniel Crosby
- Looking for pGK12
Derek Jones
- Recommendations for light level protein detection?
Kenneth J McNeil
- (none)
HARDIES at THORIN.UTHSCSA.EDU
- G50 to remove unicorporated isotope
HARDIES at THORIN.UTHSCSA.EDU
- Columns for miniprep DNA
Marty Stoltzfus
- cytosine deaminase, commercial source?
Larry Winger
- two hybrid system and differential display
Karen Browning
- homemade receipt for ECL reagents
Your Name Here
- sephadex G-50
Lauri Lintott
- Source of androgens
NEAL MUSTO
- RPA Assay kits
Rick Smoke
- Centri-sep re-use
Lisa Bibbs
- glycoprotein purification
nussbaum at neurochem.u-strasbg.fr
- need help in analysing fatty acidsmenu
Richard
- Does anyone have experience with LONG PCR?
Kelly Carpenter
- Pisia vs Baculovirus, opinions wanted
jfh
- 2 bands instead of 1
Frederick Garbrecht
- RNA tips
JoeJoe
- RPA ASSAY
Rick Smoke
- Loop-out PCR
Seth Daniel Crosby
- RNA tips
NICHOLAS THEODORAKIS
- 2 bands instead of 1
HARDIES at THORIN.UTHSCSA.EDU
- Pisia vs Baculovirus, opinions wanted
Seth Corey
- PCR SEQUENCING ADVICE
Edgar Salazar-Grueso
- .01um pore filters
Karl Voss
- RNA tips
Annette C. Hollmann
- .01um pore filters
CURTIS at UTMSI.ZO.UTEXAS.EDU
- Cycle sequencing - no product
partis
- DNA bending prediction software
Jacques Oberto
- rna sequencing
Mr. H.A. Al-Ghusein
- gel retardation
Mr. H.A. Al-Ghusein
- cytosine deaminase sources, please
Larry Winger
- E. coli origin
PhJ=van=Dalen%omb.acta%preklin at Asklepios.med.vu.nl
- MJR 96-well PTC-1 machine: suitable microtitre plates?
Martin Trick
- MJR 96-well PTC-1 machine: suitable microtitre plates?
Toby Bradshaw
- PCR with 3' mismatch
m-brazil at nimr.mrc.ac.uk
- two hybrid system and differential
mccallo at rand
- T4 lysozyme
lesley brown
- Fast thermocycler
Shiao Y. Wang
- Recipe for linear polyacrylamide
Duncan Clark
- Fast thermocycler
Douglas Rhoads
- single-step RNA method and sodium acetate
Daniel Meier
- Fast thermocycler
kofoid
- SYBR Summary-Molecular Dyna
Jeanne Loring
- Deprobing problem
Deborah Croteau
- Deletion Factory System (Gibco)
Steve Whitwill
- expression/induction depends of container
XRDPROC at Gems.VCU.EDU
- Carlisle Landel!
nishir at ohsu.edu
- SYBR Summary
Jeanne Loring
- SYBR Summary-hanzel@mdyn.co
Jeanne Loring
- Electroporation Improvements
Ben-Sapiens
- amylase and cellulase
Mark A Gallo
- Black dots on Sequence Films
James M Wahlberg
- Automated Hybridisation
r_alderton at icrf.icnet.uk
- Electroporation Improvements
John Nash
- RNA freezing
Desrochers Normand
- Macintosh Software for teaching Genetics
SPIKE
- Linear Plasmid From Borrelia
Ali Karami
- Negative Cooperativity
Louis Hom
- Precip. DNA from agarose "squeeze" ?
the End
- remove my name from the list
MAHER DERBEL
- DIG labeled probes
Kath.Nesbitt at plant.utas.edu.au
- faint bands in PCR
Xin-Hua Zhou
- wanted: 2 umole scale synthesis of ribo-oligo (4mer) for under $150
Dongxian Yue
- T-tailing for PCR product ligation
Loryn Sellner
- calibrating gilsons
Farid John Ghadessy
- Nonisotopic AA transport assays: summary
Hr Dr. S. Shapiro
- Cell cycle analysis kits
Graham Atherton
- t-tailing vector of choice
m-brazil at nimr.mrc.ac.uk
- Removal of Dna Probes
Emma Macfarlane
- expression/induction depends on container
hardies at BALIN.UTHSCSA.EDU
- Dig labeled probes
4700gbera at UMBSKY.CC.UMB.EDU
- Re. RNA Storage.
J.P. Hays
- Any softwares to draw plasmid maps?
Xin Guo
- 5' filling with Klenow
ez003450 at chip.ucdavis.edu
- takara address
SAtyam Subrahmanyam
- info about suicide vectors systemsIs2
PANIAGUA SOLIS JORGE FERNANDO
- BAC vectors
CHAZ
- Fungus in Microfuge tubes
Neeraj Jain
- Making buffer
Min Kuan
- modified oligos
Francois DRAGON
- help with protein expression
eb86336 at inet.d48.lilly.com
- faint bands in PCR
eb86336 at inet.d48.lilly.com
- artifact bands on sequencing gels
L. Fred Jerva
- Blue/white colony selection problems
eb86336 at inet.d48.lilly.com
- columns for miniprep dna
eb86336 at inet.d48.lilly.com
- Patenting of Biotechnology
eb86336 at inet.d48.lilly.com
- Subcloning PCR
eb86336 at inet.d48.lilly.com
- program for Motif identification?
Daniel S. Chen
- Internal RBS.
eb86336 at inet.d48.lilly.com
- Ligating small fragments into large vectors.
eb86336 at inet.d48.lilly.com
- 5' filling with Klenow
Martin Kennedy
- 5' filling with Klenow
Martin Kennedy
- subcloning PCR
mthomson at bscr.uga.edu
- arabidopsis RNA prep needed
Richard Dana
- Project Leader in Cell Culture
Oh Kah Weng Steve
- where to get Rapp Polymere TentaGel?
quarrell at vax.oxford.ac.uk
- Looking for Renex-30
sandra at physio.uct.ac.za
- pcr
Juan C. Mendible UCV
- Problem with inversed binding on a dot blot
kramerse at wmvx.lvs.dupont.com
- takara address
Rebecca Schall
- 5' filling with Klenow
Curt Ashendel
- Gapdh RT-PCR Probe
Dr. M.D. Jones
- Promega Taq vs. Perkin-Elmer Taq
an125249 at anon.penet.fi
- pCp (NOT the drug)
Jack M. Bernstein, M.D.
- pRIT5
JOHN NEILL
- deprobing problem
vdrover at kean.ucs.mun.ca
- lambda vectors without BglII/MunI sites
Peter Uetz
- Blood DNA
Pierre Commercon
- pcr
Robert Horton
- Blood DNA
Robert Horton
- SEQUENCING OF PCR PRODUCTS?? HMMMMMMMMMMM
russos at HAL.HAHNEMANN.EDU
- Tightly regulated E. coli Promoters
Philip Heacock
- SEQUENCING OF PCR PRODUCTS?? HMMMMMMMMMMM
Annette C. Hollmann
- PCR Product Purity Assay
VICDEF at BIOLOGY.BIOSCI.WAYNE.EDU
- Where to get ppGpp??
Hoosier Habs Fan
- PCR directly from phage
Harry Menegay
- SEQUENCING OF PCR PRODUCTS?? HMMMMMMMMMMM
Lisa Bibbs
- Northerns
Alan H. Schulman
- SEQUENCING OF PCR PRODUCTS?? HMMMMMMMMMMM
Roger Wiegand
- SEQUENCING OF PCR PRODUCTS??? HMMMMMM
HARDIES at THORIN.UTHSCSA.EDU
- TA cloning doesn't work if a certain base is at 5' end of primer?
Robert Rumpf
- In Vivo Excision of ZapII
Jon Burrows
- PCR directly from phage
Ron Kagan
- SEQUENCING OF PCR PRODUCTS?? HMMMMMMMMMMM
Joan Rowe
- Help-nucleic acids
Abdelwahab_Ahmed at UQTR.UQuebec.ca
- Concealed
clontech!CHRISC at WPSMTP.netcom.com
- Quantifying mouse IFNAR revisited...
Mary Coussons-Read
- TGF beta related
Shu-Chih Chen
- PCR primers for TCR
Loryn Sellner
- Blood DNA (Pierre C.)-
Peter
- Streptomyces PCR mutagenesis
pdxrmb at vaxa.nott.ac.uk
- pcDNA3
mbjpl at seqnet.dl.ac.uk
- Ecoli(ori)
PTJ=Willemsen%omb.acta%preklin at Asklepios.med.vu.nl
- vector lab. fax
Monique Rogard
- Dig labeled probes
HARDIES at THORIN.UTHSCSA.EDU
- RNA tips (DEPC and nucleic acid)
HARDIES at THORIN.UTHSCSA.EDU
- RNA tips
Stefan Gruenert (WCI)
- SEQUENCING OF PCR PRODUCTS??? HMMMMMM
Kris Kalra
- Qui A
ITABAJARA DA SILVA VAZ JUNIOR
- Re-diff. display problems
babalola at MAIL.SAS.UPENN.EDU
- Schneider-2 M3 medium
tahilyamani.com at syntex
- Re-using SYBR Green?
Julie Constable
- Kohara mapping membrane
kertesz at micro.biol.ethz.ch
- Frequently Asked Question (FAQ) list
Paul N Hengen
- SuperScript II RNase H- RT
Harry_Au at SOM-LRC.UCSD.EDU
- Help with PCR
Scott Wildenberg DGM-IHG
- Who has this stuff?
clontech!CHRISC at WPSMTP.netcom.com
- protein overproduction in E. coli
Patric Lundberg
- Thrmobin inhibition/removal
Patric Lundberg
- ABI sequencing artifact
Matthew Stoecker
- 16S RNA E-mail server?
James M Wahlberg
- Clostridium acetobutylicum cultures
Rod Putland
- 16S RNA E-mail server?
Niels Larsen
- Ecoli origin
D=Piket%buro%preklin at Asklepios.med.vu.nl
- question for Aussies only
LOWEN,Darren
- Cre/lox vectors
Dr Johnjoe Mcfadden
- Entrez
Graham Atherton
- Racks for Sterilin Universals?
Brian Smith
- protein overproduction in E. coli
HARDIES at THORIN.UTHSCSA.EDU
- Beware pGEM-T
Phil Emery
- Thrmobin inhibition/removal
Gene Holowachuk
- BRL Deletion Factory System
Steve Whitwill
- Any better way to get rid of MDE gel ?
gli at UCDAVIS.EDU
- Schneider-2 M3 medium
Neil Winegarden
- Mitochondrial genome size determination
Raman Sharma
- Help with RPA on tissue lysates
MUNGO at UVVM.UVIC.CA
- Recommendations for light level protein detection
Dr. D.G. Fernig
- Re-subscribe
Steve Schramm
- PCR from serum
C.J. Hussussian, MD
- Promega Cell Titer Kit
Janet De Souza
- Colony lifts - detection with biotinylated probes - background?
Paul A. Vescio
- Diff. display problems
HARDIES at THORIN.UTHSCSA.EDU
- Colony lifts - detection with biotinylated probes - background?
Peter M. Muriana
- Semlike Forest Virus
Allergan
- human genomic DNA PCR
jfh
- DNA EXTRACTION FROM PARAFIN
john
- protein overproduction in E. coli
Yasha Hartberg
- Photoaffinity labelling via radioactive ATP analogues-help
191502931
- PCR Primers for Charon 4a
David L. Osterbur
- Method for Touchdown PCR
LOGAND
- CIP or BAP
BEBLUM at ibc.unibe.ch
- Monoclonal Ab to Chlamydia
Surasakdi Wongratanacheewin
- Source of multi-filtre manifolds
Hr Dr. S. Shapiro
- RE; touchdown PCR
NEAL MUSTO
- Looking for a very tight binding Ab/epitope pair
WEDEL at vms.biochem.mpg.de
- HCHO reactivity
euverink at biol.rug.nl
- COMPETENT CELLS PROTOCOL!!!
Darren Stauth
- How to make random blunt end cuts in a plasmid?
BrianC4208
- gilson calibration .... how?
Massimo
- Rf: RE; touchdown PCR
LOGAND
- Air Thermocycler and 32P?
Amy L Teel
- Yeast Nitrogen Base??
Klara Briknarova
- COMPETENT CELLS PROTOCOL!!!
Harry Hahn
- RNA sequencing with RTase?
Harry Hahn
- Ab to RFA or RPA 70 kD subunit
Roger F. Anderson
- semliki forest virus
brett at BORCIM.WUSTL.EDU
- Measuring Intracellular Calcium Concentration
jobersta at
- Liver RNA Prep Needed
Richard Dana
- Probe Source?
rvosatka at opal.tufts.edu
- ZAP Express?
rvosatka at opal.tufts.edu
- inactivation of phosphatase
ez003450 at chip.ucdavis.edu
- TBE precipitation!
jr at dna.bio.warwick.ac.uk
- TBE precipitation!
Richard Near
- RACE Help me please!!
Paiks
- PCR without protein extraction?
Mark Siddall
- Rf: Measuring Intracellular Calcium Concentration
LOGAND
- unsubscribe
Richa Behari
- PCR from phage
Brian Fristensky
- Quantitating Radioactive label in DNA and RNA
JFrank777
- Lg Scale Fermentation
JFrank777
- Isolation of RNA from liver
Carla Hass
- Help with PCR
HARDIES at THORIN.UTHSCSA.EDU
- Restriction map of EMBL4 wanted.
rr at umesci.maine.edu
- Trizol reagent problems
Murali Srinivasan
- Quantitating Radioactive label in DNA and RNA
Klaus.Matthaei at ANU.EDU.AU
- Phage display expression library
Kah-Tong, Seow
- RNA Probe woes
D.Greeve <DAEMON>
- RNA Probe woes
Jukka Luoma
- 2-D protein gels
Jim Owens
- Linear polyacrylamide as an alternative to glycogen carrier
Duncan Clark
- Rf: 2-D protein gels
LOGAND
- RNA Probe woes
Tracy Aquilla
- Who has this stuff?
Parke K. Flick
- amber ampicillin resistance (pivx users)
Johnny D
- ABI sequencers with Genpak seq kits
Kevin Clark
- STOP ADVERTISING !!!
Stefan Kahlert
- 5 Kb by RT-PCR
Pierre Commercon
- <None>
cyto at cc.usu.edu
- Rain-X and sequencing plates
Peter J. Russell
- Ribosomal RNA prep?
RICHARD ISBRUCKER
- Posting a new newswatcher group
Labuser 1
- Microwave restriction digests
Prenom Nom
- RT-PCR GAP in HL-60
Janet E. Rubin
- Ribosomal RNA prep?
Michael van Waes
- PCR subcloning
Mel Trecker
- Ribosomal RNA prep?
Tracy Aquilla
- TK promoter in transgenic mice: expression pattern?
No One
- mouse evx 1 probe?
Rachelle Leger
- Northern blots, which is the best membrane??
Thomas Burglin
- Reusing Ethidium Bromide
Dave Carmean
- Liver RNA Prep Needed
Klaus.Matthaei at ANU.EDU.AU
- Oligo synthesisers: Millipore Expedite vs Beckman Oligo 1000
Dr. Jeremy Martinson
- Oligo synthesisers: Millipore Expedite vs Beckman Oligo 1000
Dr. Jeremy Martinson
- Quantitating Radioactive label in DNA and RNA
Klaus.Matthaei at ANU.EDU.AU
- RNA Probe woes
Klaus.Matthaei at ANU.EDU.AU
- Screening Phage Bank With Degenerate Oligo
Eric Lippay
- DIG labeling
Kath.Nesbitt at plant.utas.edu.au
- Oligo synthesisers: Millipore Expedite vs Beckman Oligo 1000
Roland J Saldanha
- UNSUBSCRIBING, BIOSCI ARCHIVES, ADDRESS DATABASE & BIOSCI FAQ
BIOSCI Administrator
- test...
Prenom Nom
- Rain-X
vdrover at kean.ucs.mun.ca
- ribosomal RNA prep
vdrover at kean.ucs.mun.ca
- Nuclear extracts
Matthias Zeiner
- Problems with 1.5 ml Eppendorf tubes
Raffaele Matteoni
- pcr yield
Mr. C.S.Wilding
- TA cloning doesn't work if a certain base is at 5' end o
Robert Rumpf
- Desperately seeking Schering Agrochemicals
LOGAND
- TA cloning doesn't work if a certain base is at 5' end o
Robert Rumpf
- DIG labeling PCR probes
Brett Beitzel
- Know of a Penicillium Library?
X-Man
- antibody to SOD
Shao-Yu Chen
- p13suc1-Sepharose
Heidi Feiler
- unitcost-rflp/pcr
Ann Baker
- DIG labeling PCR probes II
Brett Beitzel
- Info about AFLP
NICOLA LA PORTA MMKAB
- Transients-DMSO shock.
George W Chacko
- looking for Xenopus cDNA libraries
Tanya Reichert
- Genomic DNA from fish blood
Hilary Morrison
- Taq Pol and 3' primer mismatch?
Carlisle Landel
- Non radioactive Northern Blot Procedure
books at mcvax2.d48.lilly.com
- Treponema phagedenis DNA
Jerry Grandoni
- Non radioactive Northern Blot Procedure
books at mcvax4.d48.lilly.com
- Microwave restriction digests
Viraj Master
- Methods for Ascorbate Phosphatase
Thanit Pewnim
- Problems with 1.5 ml Eppendorf tubes
Anthony Palombella
- Spin-Elution and ppt. of DNA
JFrank777
- On-line source for genotypes?
Peter Horn
- Yeast Nitrogen Base??
gibbs
- PC12 cells- how to culture
T. S. Pillay
- Help- getting out 0.5 kb insert from plasmid.
Mandalaparthy
- Help- with lge scale phage prep
Mandalaparthy
- Quantitative PCR help
Sergi Ferrer
- >Degas per min & Ultrasound?
Dr. A. Rosenthal
- Fuji film sensitivity loss
g_watson at icrf.icnet.uk
- Luciferase expression variability
Graham Atherton
- Fuji film sensitivity loss
Chutney
- Amplification of plasmids NOT with Chloramphenicol
Zophonias O. Jonsson
- Recombinant ENA proteins
Jaana Ojala
- DR III, PFGE
falkj-bisance
- Looking for mol. biol. courseware
Edwin van Spronsen
- Seeking USED Cryostat
Bradley Turner
- Epitope-Tagging and EM
wang qi
- Database for tyrosine phosphorylated proteins?
T. S. Pillay
- PCR Clean up, 150bp frag???
Jon Burrows
- Cheap Peptides (sm scale)?
Chris Jones
- help with 5' race
Rachelle Leger
- Determination of genome size (Cot curves)
RiverSloth
- mLFA-3 Ab
Mr. M. Kuiper
- RNA tips
BEBLUM at ibc.unibe.ch
- T7 RNA Polymerase
Yoon Lee
- In situ question
Uwe Harmening
- Epitope-Tagging and EM
Cornelius Krasel
- RNA extraction from tissue in OCT embedding compound
Harry Menegay
- Problems with cDNA-synthesis
Mattias Zetterstrand
- Two-Hybrid: Looking for S.cerevisiae Activation-Domain Library
Seth Findley
- Radio-labelling whole cells (bacteria) protocol??
PRAMATHESH PATEL
- Glowing BLUE DNA Gels
Paul N Hengen
- Membrane protein fusion vectors? Know of any?
Peter M. Muriana
- what's the best way to clone PCR products?
jfh
- 5' RACE
Tom K. Lannom
- capillary electrophor. - sensitivity w/fluorescence??
Neil Clarke
- Blue sectors from plasmids with inserts
ez003450 at chip.ucdavis.edu
- UV Protein Scanning.
Min Kuan
- Glowing BLUE DNA Gels
Klaus.Matthaei at ANU.EDU.AU
- High background on Northern Blot
GUT at QUCDN.QueensU.CA
- study of prostate tumors
Ryan Bartel
- Luciferase Assay
Yoshiyuki Shibata
- test question/problem solving
cyto at cc.usu.edu
- Ultra-low Freezers
Brian Margolin
- Affinity column needed..
Kim Hak-Sung
- Ivitrogen Electroporator II?
Brad Nicholson
- Apotag Kit (cell death kit)
Vivien Bonazzi
- MagnaSphere paramagnetic particles (SERVA)
Peter
- Microwave restriction digests
Prenom Nom
- T4 Lysozyme summary
lesley brown
- Li-cor DNA-Sequencer
Armin Ensser
- Apotag Kit (cell death kit)
Rebecca Schall
- tritium-labelled proteins: best detection method
dacoj3 at wptemp.kvl.dk
- Epitope-Tagging and EM
Anne Spang
- PCR product cloning with DK101 vector
Dr. R.B. White
- Polypeptides
ae55
- Fuji Film Loss of Sensitivity
g_watson at icrf.icnet.uk
- seed cDNA libraries
Peta C. Bonham-Smith
- Help...can't stop RBC lysis in trout blood
vdrover at kean.ucs.mun.ca
- Ultra-low Freezers
Carla Hass
- periplasm signal sequences
Robert C. Colgrove
- retinol, cholecalciferol, vitamin B12
Victor Jimenez G.
- centrifugal vacume concentraters
jurgenson at cobra.uni.edu
- Glycogen
gc
- UV Protein Determination.
Min Kuan
- Ist strand cDNA purification.
JOEDAG
- Isolation protocol for nucleosome and splice-some
Chia Jin Ngee
- PCR and HPV
Juan C. Mendible UCV
- Catrimox.14
michele young
- Ribosomal RNA prep?
GWDGV1::KCHOWDH
- Searching for silent mutations...
David J. Austin, Ph.D.
- Affinity column - for a-amylase
camsubbu at ubvms.cc.buffalo.edu
- Problems with Promega Wizard Preps?
Mike D Amstutz
- High background on Northern blot
HARDIES at THORIN.UTHSCSA.EDU
- Mitochondrial replication
Deborah Croteau
- use of BSA in... (why air kills)
HARDIES at THORIN.UTHSCSA.EDU
- Electroporation optimal DNA conc.?
OVan-Ham
- tryptophan synth and hydroquinone
brwgor01 at uctvax.uct.ac.za
- Microwave restriction digests
John Dixon
- (none)
Zalman L. Suldan
- display phage inactivation
Ward Lab
- Broccoli question
LEES at SASK.USASK.CA
- Turner Fluorimeter
LEES at SASK.USASK.CA
- Assay of Ascorbate Sulfatase
Thanit Pewnim
- LA PCR
Nancy Templeton
- What's the best mini-prep?
RICHARD P. PHIPPS
- PCR for generating radiolabeled probes?
RICHARD P. PHIPPS
- Air cyclers and sequencing
Jun Kim
- Southern Hybridization - Strigency Question
CMS
- Cayman Chemical's EIA assay.
93huebner at wmich.edu
- Microwave restriction digests
Petr GRUZ
- Circular Dichroism
Mark Jason Logan
- LA PCR
POPOVA
- LA PCR
POPOVA
- Air cyclers and sequencing
doug rhoads
- Fast thermocycler
doug rhoads
- Turner Fluorimeter
David F. Bishop
- RNA isolation
Agnieszka Szczepek
- DEPC and PCR? A mystery!
Shahram Mori
- OKT8 (biotinylated)
George W Chacko
- cDNA Challenge*************
Shahram Mori
- ADVERTISING IN BIONET
Richard Dana
- Perkin Elmer VS Thermolyne: Recommendations?
U58563 at uicvm.uic.edu
- DNA-binding protein
WANZ at QUCDN.QueensU.CA
- gel retardation
WANZ at QUCDN.QueensU.CA
- DNA from blood
Richard Dana
- Reversible PCR termination possible?
Jim Cook
- Best Way Clone PCR Products
Dave Mahaffey
- Taq Pol and 3' primer mismatch?
Rob Slebos
- Mammalian Cell Transfection FAQ?
Goldberg Andrew
- DNA from blood
Dante Adorada
- Perkin Elmer VS Thermolyne: Recommendations?
RichWalko
- PCR w/ capillary tubes
Shiao Y. Wang
- Serum protein after repeated freeze-thaw
Paiks
- Linear polyacrylamide as an alternative to glycogen carrier
Dietmar Tietz
- plasmid minipreps
Sasha Kraev
- PCR smear
Frederick Garbrecht
- sucrose gradients and protein separation
AMRUT S BHOGLE
- calibrating gilsons
Peter M. Muriana
- Immunoaffinity Columns
Emma Macfarlane
- Culturing B lymphocytes
immdj at picr.cr.man.ac.uk
- Best methd. for PCR'in 10-15kb?
Rob Logan
- Cloning PCR products
Brett Beitzel
- >>>>URGENT : IN VITRO TRANSCRIPTION <<<<<
CJF 90/10 INSERM
- Help for IGF1, IGF2, and receptor's probes and antibodies
Jun Chen
- RBC lysis in trout blood
jd89
- GST fusion: Solution and more questions
Pavel Sova
- Reverse Northern Blot?
Dr. YL Du
- how does streptavidin bind biotin?
murali at unixg.ubc.ca
- Km of restriction enzymes
John H McDonald
- NIH netters help with ZAP
Linda Wolff
- fluorescent primers for DDRT-PCR
Roger Wiegand
- Circular Dichroism Help!
Mark Jason Logan
- need a good DNA sequencing service
Kaiser Ham Lice
- Promega on the Internet
Stephan Spencer
- Taq left at RT - gulp! -
Mark Siddall
- Help needed with transfection of Jurkat
Dominic Voon
- Extraction of DNA from agarose gels
GABRE at wwg3.uovs.ac.za
- Fax number of Clontech
Hannes Klump
- DNA from agarose gels
GLEN SHEARER
- Extraction of DNA from agarose gels
CJF 90/10 INSERM
- @#! smears on PCR ???
Frederick Garbrecht
- Source for hygromycin resistance plasmid
GWDGV1::KCHOWDH
- PCR PRIMER DESIGN HELP!!
Wenfu Wang
- Short eukaryotic promoter?
Alan Lyons
- DNA from AGPC Preps
dkim at unm.edu
- 5' RACE
swift at mcvax4.d48.lilly.com
- Electroporation of COS: How to?
g_watson at icrf.icnet.uk
- FMC SPIN BIND FOR DNA PREP?
Lisa Bibbs
- cloning problems
hl15 at hubrecht.nl
- Source for hygromycin resistance plasmid
Marion Freistadt
- cloning problems
POPOVA
- LA PCR
HARDIES at THORIN.UTHSCSA.EDU
- DNA-affinity columns
Joanne
- Aff. purified. anti hVitronectin.
Jan Svensson
- DNA-affinity columns
Joanne Tornow
- antisense oligo questions
GLEN RAFFEL
- gel retardation
WANZ at QUCDN.QueensU.CA
- protein phosphorylation leading to DNA binding
WANZ at QUCDN.QueensU.CA
- @#! smears on PCR ???
Ann M. Yezerski
- PCR from cDNA library
parin
- Oncor's Apoptag--comments, users?
jfh
- smear on PCR gels
Martin.Gossen at RUBA.RZ.ruhr-uni-bochum.de
- Tricine for electrophoresis
Francesco D'Alessandro
- extraction from agarose gels
Sasha Kraev
- Extraction of DNA from agarose gels
Jane Harrison
- FMC SPIN BIND FOR DNA PREP?
-sc2.sdsc.edu
- Taq left at RT - gulp! -
Klaus.Matthaei at ANU.EDU.AU
- PCR w/ capillary tubes
Klaus.Matthaei at ANU.EDU.AU
- WANTED: Old liquid scintillation counters
Matthew J. Combs
- Sequencing insert from Lambda gt10 vector- HELP!
Mandalaparthy
- RNA isolation failure
Lin Xiao
- Anti peptide Abs?
J.J. Baker
- Retard gel buffering conditions
grgmj at picr.cr.man.ac.uk
- Re: Taq left at RT - gulp! -
CORROCHANO PELAEZ, LUIS M.
- expression libraries
J Shepherd
- gt10 insert sequencing
Sasha Kraev
- Subtractive cDNA libraries
Peter Kulmburg
- How to transform CRL-1999?
aeschba at fmi.ch
- radiation counters
Douglas Rhoads
- DNA sequencer
b137795 at mailserv.cuhk.hk
- Biotinylated proteins
NEAL MUSTO
- Where to get shrimp alk. phosphatase?
Tracy Aquilla
- Mammalian cell transfection?
Alan Lyons
- Seq.power supply
SEPPO KAIJALAINEN MMETT
- non-radioactive cycle sequencing
klingj
- Seq.power supply
David Micklem alt.ascii-art alt.ascii-art.animation alt.asian-movies alt.astrology alt.atari-jaguar.discussion alt.atari.2600 alt.atheism alt.atheism.moderated alt.atheism.satire alt.ato alt.autos.antique alt.autos.camaro.firebird alt.autos.karting alt
- Microtiter Plates
Joe M. SandyFaulkner, Jr.
- protein fluorescence: NaI quenching???
VOSS at AIMP1.UNA.AC.AT
- Where to get shrimp alk. phosphatase?
Roger Wiegand
- Anyone using Pharmacias EasyPrep device?
Jim Woodgett
- TA cloning and making your own T-vector
txpljfg at UABCVSR.CVSR.UAB.EDU
- ultracentrifugation and proteins
AMRUT S BHOGLE
- Single Copy number plasmids
j.m.lodge at bham.ac.uk
- RAPD sequencing
palasj9579 at cobra.uni.edu
- Sequencing Workshops
Nancy Bowers
- Where to get shrimp alk. phosphatase?
NICHOLAS THEODORAKIS
- Reticulocyte Lysate
Sam Volchenboum
- Re. Southern hyb. - Stringency question
HARDIES at THORIN.UTHSCSA.EDU
- Southern hyb. - Stringency question
HARDIES at THORIN.UTHSCSA.EDU
- subscription
MBC4917 at ACS.TAMU.EDU
- RNA Precipitation with Ammonium Acetate
dkim at unm.edu
- NSF Postdocs
Joan Strassmann
- Tricine for electrophoresis
grflentk at macc.wisc.edu
- Trehalose for tissue/DNA preservation
Dave Carmean
- Where to get shrimp alk. phosphatase?
Parke K. Flick
- Ta cloning and making your own T-vector
txpljfg at UABCVSR.CVSR.UAB.EDU
- Are Ab's phosphorylated?
Louis Hom
- promega wizard kits and the 373
TSRI Core Facility
- Are Ab's phosphorylated?
Annette C. Hollmann
- Type 52 Polaroid films? Who?******
Shahram Mori
- References on the use of SHRIMP alkaline phosphatase
Thanit Pewnim
- in vivo footprinting
Steve McKinney
- 35 mm slides of gels - help
Shiao Y. Wang
- about filber digestive GENE(s)
mluo at cc.memphis.edu
- 35S-Met lifetime?
Mike Morris
- Where is the sequence of pRE4???
rosas at mcclb0.med.nyu.edu
- Questions on failed T/A cloning
LOGAND
- Microtiter Plates
Zalman L. Suldan
- 5'->3' InhibitACE
Michael van Waes
- Secondary screening after differential display?
Alan Lyons
- >>>>Help on In-Vitro Transcription<<<
CJF 90/10 INSERM
- are there rat specific primers available
Henry Simpkins
- Sol 8 Transfection?
Jody G.
- measuring mice blood pressure
carlos h. pedemonte
- secondary screening after differential display
pattonaj%phvax.dnet at sb.com
- ABI sequencing and Stratagene Taq Extender
Eric C. Anderson
- PCR from cDNA library
POPOVA
- RNA extraction from fixed tissue?
Mary Coussons-Read
- Need A journal's Address!!******
Shahram Mori
- Northern blots
Ed Stephenson
- cloning PCR products
HARDIES at THORIN.UTHSCSA.EDU
- Cycle Sequencing Question
JEMERTON at BIOTECHNET.COM
- PCR for radiolabelling probes
HARDIES at THORIN.UTHSCSA.EDU
- Detection of rRNA methylation
Donna Jensen
- Km of rest. enzymes.
HARDIES at THORIN.UTHSCSA.EDU
- Antibiotic storage and shelf-life info wanted
"Mary Colter ", BIO
- 35 mm slides of gels - help
RAMPITSCH at BCRSSU.AGR.CA
- Formaldehyde Inactivation
John Nolan
- Plasmid prep from dead agar plates
mrege at samba.cnb.uam.es
- Seeking rfa, rfb clones
mrege at samba.cnb.uam.es
- Automated Sequencers
Allergan
- gastropod mucus
Adel Rajab
- Cyclophilin (prolyl isomerase) suppliers?
pathology at mscf.med.upenn.edu
- unsubscribe
Lisa Bibbs
- Oligo probe to Coding/non-coding strand - is there a preference?
Peter M. Muriana
- Northern blot memranes, summary
Thomas Burglin
- ANTIBIOTIC STORAGE
POPOVA
- Need info: any vectors harbouring SP1 or pRB
Lee Dae-Youp
- non-radioactive cycle sequencing
Allergan
- seq. power supplies
Allergan
- Sequencing Workshops
Allergan
- microwave RE digests in microtiter plates?
schupp at nauvax.ucc.nau.edu
- sequencing workshops
Allergan
- Downward DNA transfer?
LEES at SASK.USASK.CA
- Methylene blue staining for DNA?
LEES at SASK.USASK.CA
- measuring TX/mice blood pressure
carlos h. pedemonte
- small DNA fragment recovery
David B. Stern
- 2-naphthoyltrifluoroacetate
r
- SSCP question
Frederick Garbrecht
- Hair Follicle DNA Fingerprinting
Ann Kleinschmidt
- SSCP question (cold is better)
HARDIES at THORIN.UTHSCSA.EDU
- PCR primer design (r. site exten.)
HARDIES at THORIN.UTHSCSA.EDU
- Sequence of the shuttle vector pRE4
rosas at mcclb0.med.nyu.edu
- Phosphoroimagers vs Instantimager Experiences Saught
Roland J Saldanha
- small DNA fragment recovery
Klaus Salger
- William D. Huse / Ixsys, Inc -- San Diego, CA
merlin
- Downward DNA transfer?
Ron Kagan
- Silver Staining of GT gels
Lisa A. Megna
- Adress for Dr. Moshe Oren, Weizmann Ins
Pearse Ward
Last message date:
Fri Sep 30 23:03:22 EST 1994
Archived on: Sun Mar 6 20:15:28 EST 2005
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