Protocol for PCR straight from yeast colony?

Susan M. Sullivan susans at student.msu.edu
Sat Apr 1 11:39:00 EST 1995


I need to verify if the plasmids that I transformed into my S. cerevisae
are correct.  I know that it is possible to pick a colony of yeast, lyse
it, and PCR amplify a fragment of DNA using the DNA retrieved from the
yeast colony as the template.  However, I do not have a protocol for this
procedure.  If anyone knows a protocol or where to find it please let me
know. 

Thanks

John Stebbins



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