Thioredoxin fusion expression system

I-Jen Huang gr8bn at cc.usu.edu
Sat Apr 1 16:46:44 EST 1995


In article <kathryn_graham-310395104013 at mac30.biochem.ualberta.ca> 
kathryn_graham at darwin.biochem.ualberta.ca (Kathryn Graham) writes:
>Has anyone tried Invitrogen's E. coli thioredoxin fusion expression plasmid>
pTrxFus? Does it really generate soluble protein? How well does the>
enterokinase cleavage work?

I have tried this system to express a viral protein.  The expressed fusion 
protein is still insoluble even that was done at 15 C. SO I do not have any 
suggestion here.  I think that it all depents on the protein, which you are 
working with.

I. Huang
Gr8bn at cc.usu.edu



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