Direct sequence of genomic DNA

schupp at nauvax.ucc.nau.edu schupp at nauvax.ucc.nau.edu
Mon Apr 3 10:48:59 EST 1995


Kazhuhiro,

        Amersham Life Science has a single tube PCR-Sequncing kit that
involves amplifying the desired fragment to be sequenced then adding ExonucleaseI and Shrimp Alkaline Phosphatase to remove excess primers, single stranded
DNA and dNTPs.  This supposedly leaves the fragment ready to be sequenced in
less than 60 minutes.

Jim Schupp
Northern Arizona University
Biology Dept.
Flagstaff, AZ  USA



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