unexplained insert from PCR clone?
szcooley at dale.ucdavis.edu
Mon Apr 3 10:41:10 EST 1995
Steve Bryson (steve at gene4d.med.utoronto.ca) wrote:
: I've cloned a gene from a human cDNA library by PCR. The sequence
: indicates a 14 b.p. insert relative to the published sequence.
: Consequently, a stop codon is introduced immeadiately following the
: insertion. Any ideas as to the source of this insertion mutation?
: Steve Bryson
: Dept. of Biochemistry
: University of Toronto
: E-mail: steve at gene4d.med.utoronto.ca
How about a pseudogene?
More information about the Methods