unexplained insert from PCR clone?

Michael Cooley szcooley at dale.ucdavis.edu
Mon Apr 3 10:41:10 EST 1995


Steve Bryson (steve at gene4d.med.utoronto.ca) wrote:
: I've cloned a gene from a human cDNA library by PCR. The sequence 
: indicates a 14 b.p. insert relative to the published sequence.
: Consequently, a stop codon is introduced immeadiately following the
: insertion. Any ideas as to the source of this insertion mutation?

: Steve Bryson 
: Dept. of Biochemistry
: University of Toronto
:  E-mail: steve at gene4d.med.utoronto.ca
:    

How about a pseudogene?





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