How to make a-32P-GMP

Karl Fischer kfischer at
Mon Apr 3 09:05:22 EST 1995

In article <3lm9tg$hgb at>,
fb4a051 at (Friedemann Janus) wrote:
> I'm trying to crosslink a protein to GMP, but a-32P-GMP is not 
> comercially available. Therefore I'm looking for a way to make GMP out of 
> a-32P-GTP. I'm thinking about using phosphodiesterase. Has anybody done 
> something similar ? Any tips ?
> Thanks in advance

You could try reacting the labelled triphosphate with apyrase (see Sigma
cat.) and then resolving the products by TLC or HPLC prior to isolation.

Just my 2¢ worth.


Karl Fischer
kfischer at
tyr-2 at

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