How to make a-32P-GMP

Karl Fischer kfischer at gpu.srv.ualberta.ca
Mon Apr 3 09:05:22 EST 1995


In article <3lm9tg$hgb at rzsun02.rrz.uni-hamburg.de>,
fb4a051 at rrz.uni-hamburg.de (Friedemann Janus) wrote:
 
> I'm trying to crosslink a protein to GMP, but a-32P-GMP is not 
> comercially available. Therefore I'm looking for a way to make GMP out of 
> a-32P-GTP. I'm thinking about using phosphodiesterase. Has anybody done 
> something similar ? Any tips ?
> Thanks in advance

You could try reacting the labelled triphosphate with apyrase (see Sigma
cat.) and then resolving the products by TLC or HPLC prior to isolation.

Just my 2¢ worth.

Karl

-- 
Karl Fischer
kfischer at gpu.srv.ualberta.ca
tyr-2 at bones.biochem.ualberta.ca



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