Protocols to quantitate westernblots
rawlings at silcom.com
Tue Apr 4 11:37:22 EST 1995
In article <136157 at cup.portal.com>, Zekeman at cup.portal.com (Enrico Ami-tox
> Hello all, I am searching for methods that deal with quantitation of immuno-
> blots. I have plenty of software but no real protocol. All of the software
> I have alludes to measuring blots but non actually commits a logical protocol
> for it. Any ideas, thought, or comments on this. I figure the method will
> only be semi-quantitative considering all of the variables that are involved.
> Any help would be grateful.
I worked out a protocol using I-125 labeled secondary antibody, followed
by visualization with a phosphorimager. If you have a source of puified
antigen, of known concentration, you can use that as a standard on the
blot. I believe such a protocol can be quantitative.
Jeff Rawlings Internet Consultant
rawlings at silcom.com and
rawlings at cco.caltech.edu Research Biologist
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