Ligation of large plasmids

R Daines rdaines at aol.com
Tue Apr 4 21:19:49 EST 1995


You shouldn't have a problem making a plasmid of the size you describe...a
few more details would help though.  What are the ends of the two inserts,
is the sticky end fragment the 3 KB one that seems to go in OK?  If one is
a double blunt are you alkaline phosphatase treating the backbone plasmid
to prevent recircularization?

Richard



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