Transfection question

Soeren Jensby Nielsen sjn at biobase.dk
Wed Apr 5 06:44:21 EST 1995


Hi

In most of the articles that I have read concerning deletion analyses of
promoters/enhancers the authors transfect a fixed AMOUNT of reporter plasmid
together with an internal transfection standard. Now if you're interested in
examining say 2 kb of promoter/enhancer in this way and your full-length 
plasmid is say in the order of 6 kb, then you actually transfect at least 30%
more moles of plasmid in the case of the fully deleted fragment if you stick
to a fixed AMOUNT of reporter plasmid. Now is this not conceptually wrong?

Soeren




More information about the Methods mailing list