Positive selection vectors

Ron Laby rjl2 at cornell.edu
Thu Apr 6 12:58:35 EST 1995


Hello,

Recently I saw a posting here requesting an lacZ alpha-complementing galE-
 E. coli strain, to be used for positive selection of plasmids w/inserts,
in the presence of phenylgalactoside.  While I can't answer that question
directly, when looking through the databases, I came across a number of
references to vectors constructed to enable positive selection of
insertion-containing plasmids.  The references follow, for anyone who is
interested:

Gene 148:71
Gene 143:145
J. Biotechnol. 33:15
Gene 138:109
Meth. Enz. 216:457
Gene 68:119


Ron



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