PCR from phage suspension

Steven Goldberg goldberg at bms.com
Thu Apr 6 11:51:07 EST 1995


I have a number of cDNA clones in gt11 that I want to screen using
PCR.  Single plaques were picked and diluted into 0.1 ml SM buffer.
Assuming the SM buffer doesn't interfere with PCR, how much of my
phage suspension should I use in a standard 0.1 ml reaction?  Or are 
there any alternative protocols that someone can suggest?

Thanks for any help.

Steve Goldberg



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