Conjugation/labeling using carbodiimide-HEL
carlos h. pedemonte
cpedemonte at UH.EDU
Thu Apr 6 11:33:06 EST 1995
>I am currently involved in a research project and I need your help! Do you
>do cojugations, labelings, etc. to proteins using water soluble
>carbodiimides, especially EDC? If so, or if you know of procedures for
>doing so, would you please take a few moments and either send me your exact
>procedure or a reference to the one you use? I am especially interested in
>hearing from those of you who are linking amine functions to Asp or Glu in a
>protein. Please include in your reponse the following:
>-time of reaction;
>-concentration of EDC or WSCDI used;
>-concentration of protein/labeling agent; and
>I am posting this in the hope of getting as wide a variety of procedures as
>possible. I will send to all who repond a summary of the procedures I get,
>as well as a summary of the results of our experiments (NOTE: I am not
>selling anything, nor will I be!). Thanks!
I have used EDC to bind nucleophiles to carboxylates of proteins. If you
think that my experience can be useful, you can read the methods in the
Pedemonte and Kaplan (1986) J. Biol. Chem. 261, 3632-3639.
Pedemonte and Kaplan (1986) J. Biol. Chem. 261, 16660-16665.
Tell me if you need some more information.
carlos h. pedemonte Phone: 713-743-1228
University of Houston 713-743-1211
Dept. of Pharmacology Fax: 713-743-1229
Houston, TX 77459-5515 e-mail: pedemonte at jetson.uh.edu
More information about the Methods