Luciferase experiments Help!!!!

[Tina Van Dyk]

In article <Reon.3.2F83D793 at INFRUIT1.AGRIC.ZA>, Reon at INFRUIT1.AGRIC.ZA
(Reon Brand ) wrote:

 
> 
> Anyone out there ever used decanal (n-decyl aldehyde) as a substrate for 
> luciferase? I've got the stuff, but don't know how to use it. I also cannot 
> find any articles that are usefull.


We use nonanal rather than decanal.  It is a liquid and hence easy to add
directly to medium.  Nonanal is a good substrate for the luciferase from
Vibrio harveyi, Vibrio fischeri and Xenorhabdus luminescens (see Meighen.
1991. Microbiological Reviews. 55: 123-142)

Tina Van Dyk
DuPont Co. 
Environmental Biotechnology 
vandyk at esvax.dnet.dupont.com