Primer Tm for sequencing

Chris Silvia icagen at
Thu Apr 6 20:21:51 EST 1995

  What is thought to be the optimal characteristics for
sequencing primers, specifically regarding Tm, GC clamping,
and primer self-annealing?  I use alkaline conditions to 
denature sequencing template in the standard Sequenase
way, but the availability of primer design software has
made me think that a more rational approach to sequencing
primer design is possible instead of the usual hit-and-miss
eyeball method.


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