lacZ

benedik at uh.edu benedik at uh.edu
Thu Apr 6 16:57:57 EST 1995


In article <sbv-060495102100 at path22304.path.cwru.edu>
sbv at pop.cwru.edu (Scott Vande Pol) writes:

> 
> Does anyone know if the last 20 codons of lacZ (ie., at the EcoR1 site) are
> necessary for galactosidase activity?  I want to use this R1 site at the 3'
> end for cloning but want enzyme activity preserved.
> 
> Thanks in advance!
> 
> -- 
> Scott Vande Pol
> Case Western Reserve University
> Department of Pathology
> Cleveland Oh. 44106
> sbv at pop.cwru.edu

If you insert a fragment into the EcoRI site you lose lacZ activity.

Michael Benedik               benedik at uh.edu
Biochemical Sciences
University of Houston
Houston, TX 77204-5934



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