benedik at benedik at
Thu Apr 6 16:57:57 EST 1995

In article <sbv-060495102100 at>
sbv at (Scott Vande Pol) writes:

> Does anyone know if the last 20 codons of lacZ (ie., at the EcoR1 site) are
> necessary for galactosidase activity?  I want to use this R1 site at the 3'
> end for cloning but want enzyme activity preserved.
> Thanks in advance!
> -- 
> Scott Vande Pol
> Case Western Reserve University
> Department of Pathology
> Cleveland Oh. 44106
> sbv at

If you insert a fragment into the EcoRI site you lose lacZ activity.

Michael Benedik               benedik at
Biochemical Sciences
University of Houston
Houston, TX 77204-5934

More information about the Methods mailing list