ammonium sulfate cuts

Seth Corey corey at MED.PITT.EDU
Sun Apr 9 21:47:54 EST 1995

I want to ppt out an IgM from tissue culture media.
Will (NH4)2SO4 work?
The description in Harlow or the Red Book seems sketchy.
I tried to make saturated (NH4)2SO4 - 76.1 g/100 ml. But there was a layer of
crystals. Do you use the upper layer?
Thanks for any tips.

Seth Corey
Univ of Pittsburgh

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