reprobing dna dot blots

a.buchan a.buchan at bham.ac.uk
Tue Apr 11 12:18:02 EST 1995


We are using Amersham Hybond N membranes for the detection of HPV DNA by
dot blot with Boehringer DIG labelling and chemiluminescent kit.  The probe
is full length HPV DNAs and the targets are full length HPV DNAs.
We wish re-probe but the first probe is resistant to complete removal with
either 0.2N NaOH/0.1% SDS or 0.1%SDS at >95C.
Any suggestions?
Sandy Buchan(a.buchan at bham.ac.uk) 



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