Soluble fusion proteins

Wendy Bedale bedal001 at mc.duke.edu
Tue Apr 11 16:59:40 EST 1995


In article <3mdbgr$45d at griffin.ccc.nottingham.ac.uk>, Simon Dawson
<mbxspd at unicorn.nott.ac.uk> wrote:

 Has anybody out there tried Invitrogens new 'ThioFusion' kit which 
> claims to make proteins expressed in E. coli soluble?

I have tried it, and my protein was STILL insoluble. Boo hiss.  But my
impression from Invitrogen was that almost all thioredoxin fusion proteins
that people had made to date were at least partially soluble.



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