Q: Minimum length for homologous rec. in E. coli

Weiss biochem at clio.rz.uni-duesseldorf.de
Tue Apr 11 09:04:09 EST 1995


Hi out there!

Just a short question: I plan to construct an in frame deletion in an 
E. coli gene. What is the minimum length of the flanking regions for 
efficient homologous recombination?
Any literature or personal experience would be helpful!

Thanks in advance!

Annette Berger



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