alternative to co-IPs?

Noam Harel Harel_n at a1.mscf.upenn.edu
Wed Apr 12 15:06:22 EST 1995


	I would like to be able to co-immunoprecipitate two
 proteins transiently transfected into Hela cells, but realize that transfection efficiency
and low expression levels may be beyond the limits of detection. Does anyone know if
it is possible to lyse transfected cells under non-denaturing conditions, then
run the lysate on a non-denaturing PAGE followed by a Western to detect
potential complexes containing the protein of interest and other proteins
to which it binds?  Would this method be more sensitive for detecting the presence
of two non-abundant proteins in a bunch of transfected cells?



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