GST fusion vector for cloning Pst1 fragment

rpgrant at molbiol.ox.ac.uk rpgrant at molbiol.ox.ac.uk
Wed Apr 12 12:20:31 EST 1995


In article <D6uCFn.7uq at news.cis.umn.edu>, shin at cis.umn.edu (Shin Enomoto) writes:
> Is there a GST fusion vector suitable for cloning a Pst1 fragment.  We 
> are looking for a vector that has NsiI site in the polylinker.
> 


Unlikely, but you could always make your own.  Design the polylinker you want, 
get your friendly DNA oligo synthesizer to make it (need fwd and rev strands), 
anneal, ligate, presto!

Richard



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