Help! SDS-PAGE from Proteins dissolved in 6m GuHCl?

Stephan Witte Stephan.Witte at
Thu Apr 13 07:50:26 EST 1995

Hi over there,

I want to analyse protein samples dissolved in 6M GuCl by SDS-PAGE.
(SDS precipitates with GuHCl, and all the bands are smeary) 
Does anyone know a protocol for doing this without the fussy removal of
the GuHCl by dialysis or chromatography?
My samples are very small and diluted, so 10fold dilution with suitable
doesn«t work.

Thank you very much

Stephan Witte
Dept. of Immunology
University of Constance
stephan.witte at 

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