Help! SDS-PAGE from Proteins dissolved in 6m GuHCl?

Stephan Witte Stephan.Witte at uni-konstanz.de
Thu Apr 13 07:50:26 EST 1995


Hi over there,

I want to analyse protein samples dissolved in 6M GuCl by SDS-PAGE.
(SDS precipitates with GuHCl, and all the bands are smeary) 
Does anyone know a protocol for doing this without the fussy removal of
the GuHCl by dialysis or chromatography?
My samples are very small and diluted, so 10fold dilution with suitable
buffer 
doesn«t work.

Thank you very much

Stephan Witte
Dept. of Immunology
University of Constance
stephan.witte at uni-konstanz.de 



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