Help! SDS-PAGE from Proteins dissolved in 6m GuHCl?
Stephan.Witte at uni-konstanz.de
Thu Apr 13 07:50:26 EST 1995
Hi over there,
I want to analyse protein samples dissolved in 6M GuCl by SDS-PAGE.
(SDS precipitates with GuHCl, and all the bands are smeary)
Does anyone know a protocol for doing this without the fussy removal of
the GuHCl by dialysis or chromatography?
My samples are very small and diluted, so 10fold dilution with suitable
Thank you very much
Dept. of Immunology
University of Constance
stephan.witte at uni-konstanz.de
More information about the Methods