Differential Display

Doreen E. Osterholm doreeno at .bcm.tmc.edu
Wed Apr 12 17:01:32 EST 1995


Not all TAQ polymerases are alike...at least in PCR.
A lab I used to work in did a test using the same bufferes and
templates but varied the Taqs using four major brands, and the
Perkin-Elmer Taq did work the best relative to the others. The
buffer was homemade so nothing special was added to enhance a
particular brand. I became a believer. Who wants to mess around
with false negative results?

Doreen Osterholm



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