Cutting close to ends with Tsp509 I

John H McDonald mcdonald at
Thu Apr 13 13:12:52 EST 1995

Does anyone know if Tsp509 I has problems cutting at sites that are a few 
bases from the end of a fragment?  It's not one of the enzymes listed in 
the appendix to the NEB catalog on this subject.  I ask because a student 
is having trouble getting two Tsp509 sites that are eight bases apart to 
cut, when a couple of other Tsp509 sites in her PCR product have cut to 

John H. McDonald
Department of Biology
University of Delaware

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