NIH 3T3 electroporation protocol

U58283 at U58283 at
Fri Apr 14 11:15:39 EST 1995

Hi, netters!

Can anybody give me a protocol for transfection of NIH 3T3 cells
by electroporation (BIO-RAD Gene Pulser). Basically I'm looking for
voltage-capacitance parameters for these cells - I do e/p for PC12
and just don't want to waste time for optimization of conditions.
Journal references are just fine.

Thanks a lot in advance!


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