Q: Minimum length for homologous rec. in E. coli
dkim at nmsu.edu
Thu Apr 13 12:03:15 EST 1995
Re: homologous recombination in E. coli:
There have been several articles on the technique "recombinant circle
PCR" in BioTechniques (1993, 1994, I think). You might want to do a
search through BioTechNet. Just Telnet to biotechnet.com and login as
BIOTECH. Password is "technique". This will give you the
public-accessible features of biotechnet, including a database of all
BioTechniques articles. The interface is all menu-driven, so it should
not be a problem to run it.
I believe the length of homologous ends used in recomb. cir.pcr is about
20 bp minmum.
dkim at verdi.nmsu.edu
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