Carrier for EtOH precipitation: oligo-polyacrilamide?

Hiroyoshi Ohba ohba at YL05HP.TB.NODA.SUT.AC.JP
Fri Apr 14 06:29:44 EST 1995


Dear Netters;
        For ethanol precipitation of DNA fragments, I have been using 1
microgram of glycogen as a carrier.  However, someone suggested that such
amount of glycogen reduces 30% of activity of ligase.  If I like to avoid
this reduction, I should use oligo-polyacrilamide instead.  How can I make
this and how should I use this in EtOH ppt?  A local reagent company called
Nippon Gene sells carrier which does not interrupt any enzymatic reaction. 
This looks viscous and I guess this is oligo-polyacrilamide solution.  A
bial of this carrier for 200 precipitations costs 15,000 yen equivalent to
176.5 US$.  OOOPs!  Don't say buy this.  Thanks for your advise.
regards,
Hiroyoshi
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Hiroyoshi Ohba, Ph.D.
Laboratory of Immunology,
Department of Biological Science & Technology,
Science University of Tokyo
2641 Yamazaki, Noda, Chiba 278 Japan                               
Tel: 81-471-24-1501 ext. 4429      FAX: 81-471-25-1841             
e-mail: ohba at yl05hp.tb.noda.sut.ac.jp
(domain name is y-el-zero-5hp not y-one-owe-5hp, sorry) 
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