Protein electroporation. Need advice.
grtfulned at aol.com
Sun Apr 16 22:27:37 EST 1995
We would like to load a recombinant protein into HeLa cells. I think
electroporation would be a good way to do this, but have not tried it.
Does anyone have any experience in doing this? We have a BioRad
electroporator. Should we use the same settings as for DNA
electroporation? Does anyone know good settings for protein
electroporation? Can we cotransfect a plasmid a the same time to serve as
a transfection control? Any help greatly appreciated? Thank you. Ned
Landau, Ph.D., Aaron Diamond AIDS Research Center
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