Help! RNA extraction from liver...
zu03388 at uabdpo.dpo.uab.edu
Mon Apr 17 20:29:22 EST 1995
Andrew JACKSON <ajackson at cs.mcgill.ca> wrote:
>I seem to be having a problem extracting RNA from rat Livers. The RNA
>produces bright streaks on 1% agarose gels (EtBr stained). The 28 & 18S
>rRNA bands are merely brighter bulges in the streak.
>The other tissues I have extracted RNA from seem OK. I get large yields
>and little streaking.
>Can anyone help me? Or at least tell me why this is occurring in the
>liver but not in other tissues?
It's probably because liver is a very active tissue. We use Triazol
in our lab to isolate RNA from developing gonads, and see the same
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