PCR mutagenesis?

Paul N Hengen pnh at fcsparc6.ncifcrf.gov
Mon Apr 17 13:00:56 EST 1995


 In article <dhs3-170495113215 at 132.236.171.145> dhs3 at cornell.edu
 (daniel h schott ) writes:

: I want to make a PCR-random-mutagenesis by adding a smaller concentration 
: of one of the nucleotides. Does anyone has a protocol or a reference for 
: such a method? Thanks in advance,

| Eckert, K.A. and T. A. Kunkel. (1990) High fidelity DNA synthesis by
| Thermus aquaticus DNA polymerase. Nuc. Acids Res. 18:3739- 3744.
|
| Leung, D.W., E. Chen and D. V. Goeddel. (1990) A method for random
| mutagenesis of a defined DNA fragment using modified polymerase chain
| reaction. Technique (A. J. Cell. Mol. Biol.) 1:11- 15
|
| Muhlrad, D. and R. Parker. (1992) A rapid method for localized mutagenesis
| of yeast genes. Yeast. 8:79- 82
|
| Cadwell, R. C. and G. F. Joyce (1992) Randomization of genes by PCR
| mutagenesis. PCR Methods and Applications 2:28- 33 (Cold Spring Harbor
| Laboratory)

The ones that I have are:

@article{Zhou1991,
author = "Y. Zhou",
title = "Random mutagenesis of gene-sized {DNA}
molecules by use of {PCR} with {{\em Taq}} {DNA} polymerase",
journal = "Nucl. Acids Res.",
volume = "21",
pages = "6052",
year = "1991"}
 
@article{Spree1993,
author = "J. H. Spree",
title = "Efficient random mutagenesis method with
adjustable mutation frequency by use of {PCR} and {dITP}",
journal = "Nucl. Acids Res.",
volume = "21",
number = "3",
pages = "777-778",
year = "1993"}

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