harold.drabkin at channel1.com
Sun Apr 16 23:01:00 EST 1995
Subject: HELP-polyacrylamide gels cracking
J>acrylamide (with 7 M urea) gels that are 0.8 mm
J>thick (twice the thickness of my sequencing gels). Each time I have tried to
J>dry them they crack like shattered glass!!!!
J>Someone out there must know the trick to drying high percentage gels that
J>are 0.8 mm thick. Help Help
J>I have tried drying at 50 or 60 C, but the gels take forever to dry and still
I often dry down 0.75mm gels; not that far off. I fix the gel in the
same solution that I use for doing sequencing gels (10% Acetic acid, 10%
methanol), then dry down. I've done 8-even 20% this way.
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