Need help with Long PCR

Mike Dalrymple dalrymple at pplros.demon.co.uk
Tue Apr 18 02:45:42 EST 1995


In article <3mrmcc$1fo0 at news.doit.wisc.edu> Sam Henderson,
shender at macc.wisc.edu writes:
> I have been trying to amplify an 11 kb fragment from a
>plasmid using Stratagenes Taq extender. My primers are both
>33 nts each. I do the following PCR program.
>

Not really a solution but an alternative,... I tried the Stratagene
system without
success but got the Perkin-Elmer XL PCR kit to work FIRST TIME with 7, 9
and
16.8Kbp templates (all on the same gel, it looked great!!). Unfortunately,
I've not been able to clone the damn things yet!!

Mike Dalrymple



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