mfanning at LAMAR.COLOSTATE.EDU
Tue Apr 18 18:23:40 EST 1995
try perkin elmer dNTPs. We use these in our lab and have not had any
On Wed, 12 Apr 1995, Brick Ayola wrote:
> > In article <3mgvn8$67q at solaris.cc.vt.edu>, John McQuiston <dna at mail.vt.edu>
> > wrote:
> > > Anyone had problems with Promega's dNTP's?
> > > We've had three bad batches this last year and lost about 4 - 6 weeks
> > > each time trying to figure out what the hell is going on.
> > > Any suggestions for a better supplier?
> > > I've heard Gibco/BRL's are pretty good.
> On Wed, 12 Apr 1995, PJOB wrote:
> > Do you have the lot number of the bad batch? Please post if you do!
> On a similar note, I had problems with dNTP's from Pharmacia. PCR results
> would be negative if a stock soln containing all 4 dNTP's were used, but
> for some reason was OK if each dNTP was added separately. I noticed in
> Perkin-Elmer's PCR protocol that they recommend adding each dNTP
> separately or a *freshly* made dNTP mix. What happens to a dNTP mix
> stored long term? Does it increase degradation or spontaneously
> self-assemble? If so why not individual dNTP's?
More information about the Methods