Help! RNA extraction from liver...

Cathy Aversa aversa at bms.com
Tue Apr 18 17:00:40 EST 1995


In article <9504172139.AA17924 at mrcs1>, jfs at WH.BAYER.COM wrote:

> Andrew and netters worldwide-
> I understand it is the liver
> glycogen that is causing your
> problems.  I have used the GIBCO
> reagent Trizol to purify liver
> RNA with success.  Check it out.
> Re-purify dirty samples when necessary.
> 
> Jean Schaefer
> jfs at wh.bayer.com
> Bayer Research Center
> W. Haven, CT

You might also consider resuspending your RNA in formamide (ref.  NAR 20:
3791-92, 1992.)  I do this routinely with many different tissues, some more
susceptible to degradation than others, with great success.  Formamide is
also compatible for use in the loading buffer for formaldehyde RNA gels,
and samples can be stored at -20oC rather than -80oC.  Good luck!

Cathy Aversa
aversa at bms.com



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