DNA Storage?
L. Scot Bastian
yacman at homer22
Wed Apr 19 10:22:51 EST 1995
Howdy,
I have been debating the best way to store plasmids and
oligonucleotides. In the past, I have stored DNA at -20, but recently I
have become concerned about the frequent freeze-thaw cycles that might
cause mechanical shear, particularly to the plasmids as opposed to the
oligos. For the last several months, I have been storing all my DNA at
+4. It is convenient to not have to thaw the DNA every time I use it. Now
I am having second thoughts, I'm not having any obvious problems
with degradation, but I wonder if I'm going to have bugs growing in my
preps. After all, DNA is a sugar. Comments? Experiences?
L. Scot Bastian
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