detergent compatibility of mAb Immunoaffinity purification?

ANDREI POPOV ANDREI.POPOV at bbsrc.ac.uk
Wed Apr 19 18:32:07 EST 1995


Hi Robert,
I would try to increase salt.
0.5-1 M NaCl might be enough.
Another thing, maybe you elution stuff 
denatures the protein completely?
Urea is very helpful sometimes to elute/renature
proteins.

best wishes
Andrei




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