Apoptosis - DNA ladder method

Christian Seelos christian.seelos at univie.ac.at
Tue Apr 11 07:48:31 EST 1995


"Juergen Sasse (Path)" <js1 at mole.bio.cam.ac.uk> wrote:
>
> 
> Can anybody provide an easy and reliable method for preparing
> DNA ladders of apoptotic cells? Using my current method I have
> problems with the genomic DNA of control cells because the high
> molecular-weight DNA tends to crawl out of the gel slots and
> gives smears. On the other hand, purifying away soluble fragments
> from the HMW DNA leaves blank control lanes (because there is low
> apoptosis background in my control cells) which is not really 
> satisfying.
> Your help is much appreciated,
> Christian
> 




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